食品科学 ›› 2006, Vol. 27 ›› Issue (11): 262-266.

• 工艺技术 • 上一篇    下一篇

节点文献糙米皮层中抗营养因子酶解条件的研究

 张瑞宇,  曾金林   

  1. 重庆工商大学环境与生物工程学院
  • 出版日期:2006-11-15 发布日期:2011-11-28

Study on Conditions of Enzymatic Hydrolyzing Anti-nutrition Factor in Brown Rice

 ZHANG  Rui-Yu,   Zeng-Jin-Lin   

  1. College of Environmental and Biological Engineering,Chongqing Technology and Business University,Chongqing 400060,China
  • Online:2006-11-15 Published:2011-11-28

摘要:  针对影响糙米可食用性的主要抗营养因子(ANF)——纤维素与植酸,直接外源添加纤维素酶和植酸酶作用于糙米皮层,使其分解,对影响两种酶酶解作用的主要反应条件:温度、pH、酶用量、酶解时间分别进行单因子试验,在此基础上,按L9(34)设计,通过正交试验分别对酶解环境进行了优化。结果表明:纤维素酶酶解优化条件为温度55℃,pH3.5,酶用量10ml,酶反应时间90min;植酸酶酶解优化条件为温度55℃,pH2.5,酶用量15ml,酶反应时间150min。同时使用纤维素酶和植酸酶可有效地降低糙米皮层纤维素与植酸含量,在与单一酶酶解效果相当的情况下,明显缩短了酶解时间。

关键词:  , 糙米, 抗营养因子, 纤维素酶, 植酸酶

Abstract: Aiming at cellulose and phytic acid,the anti-nutrition factors(ANF),existing in brown rice which make brown rice unsuitable for eating,the cellulase and phytase were directly put in brown rice in order to hydrolyze the cellulose and phytic acid in cortex of brown rice. The main factors including temperature,pH,consumption of enzyme solution,hydrolyzing time were respectively studied for each enzyme. Based on this,the orthogonal tests were conducted to optimize the reacting conditions. The results showed that the suitable conditions with cellulase were pH 3.5,10ml enzyme solution,at 55℃ for 90min,which for phytase were pH 2.5,15ml enzyme solution,at 55℃ for 150min. On the optimized conditions,phytic acid and cellulose in the cortex could be effectively decreased by using two enzymes together,meanwhile the hydrolyzing time was markedly shortened on the similar hydrolyzing results.

Key words: brown rice, anti-nutrition factor, cellulase, phytase