食品科学 ›› 2006, Vol. 27 ›› Issue (7): 159-162.

• 工艺技术 • 上一篇    下一篇

酶法提取元宝枫叶绿原酸的新工艺研究

 胡青平, 徐建国, 李琪, 陈五岭   

  1. 西北大学生命科学学院; 山西师范大学食品工程系;
  • 出版日期:2006-07-15 发布日期:2011-09-29

Study on New Extraction Technology of Chlorgenic Acid in Acer truncatum Bunge Leaf with Enzyme Treatment

 HU  Qing-Ping, XU  Jian-Guo, LI  Qi, CHEN  Wu-Ling   

  1. 1.College of Life Science, Northwest University, Xi′an 710069, China; 2.Department of Food Engineering, Shanxi Normal University, Linfen 041000, China
  • Online:2006-07-15 Published:2011-09-29

摘要: 以元宝枫叶为原料,对酶的种类进行了筛选,然后,通过单因素试验、正交试验和方差分析确定了酶法提取绿原酸的最佳操作条件。结果表明,采用纤维素酶提取元宝枫绿原酸效果较好;确定的最佳操作参数为:纤维素酶添加量0.015%、pH值为4.5、酶解温度50℃、酶解时间1.5h。

关键词: 元宝枫, 绿原酸, 纤维素酶, 提取

Abstract: Taking Acer truncatum Bunge leaves as the raw material, the best enzyme was screened from different enzymes for extracting the chlorgenic acid. The single factor tests, orthogonal experimental designs and variance analyses were applied to optimize manipulation parameters of enzymes in the extracting technology. The results showed that the cellulase possessed the optimum effect to extract chlorgenic acid among the other enzymes. The optimum manipulation parameters are: cellulase dosage 0.015%, pH value 4.5, 50℃ and extraction time 2.2h.

Key words: Acer truncatum Bunge, chlorgenic acid, cellulase, extraction