食品科学 ›› 2007, Vol. 28 ›› Issue (10): 391-395.

• 生物工程 • 上一篇    下一篇

酿酒酵母生产谷胱甘肽的培养条件的研究

 郑丽雪, 胡耀辉, 齐斌   

  1. 吉林农业大学食品工程学院; 常熟理工学院生物与食品工程系 吉林长春130118; 吉林长春130118; 江苏常熟215500;
  • 出版日期:2007-10-15 发布日期:2011-11-22

Optimization of Culture Conditions for Glutathione by Saccharomyces cerevisiae

 ZHENG  Li-Xue, HU  Yao-Hui, QI  Bin   

  1. 1.School of Food Engineering, Jilin Agricultural University, Changchun 130118, China; 2.Department of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2007-10-15 Published:2011-11-22

摘要: 本实验对酿酒酵母(Saccharomyces cerevisiae)CS10515产谷胱甘肽的培养条件进行了初步研究,确定了其最佳培养基组成为:葡萄糖为5%、酵母膏1.2%、硫酸铵0.6%、硫酸镁0.02%、磷酸二氢钾0.1%;当发酵液初始pH为6.0、装液量50%~60%、振荡速度300r/min时,经过24h振荡培养后,谷胱甘肽的产量为90.20mg/L。

关键词: 谷胱甘肽, 酿酒酵母, 生物量, 发酵

Abstract: The production condition for glutathione by Saccharomyces cerevisiae CS10515 was investigated. The results showed that the medium composition was that, glucose 5%, yeast extract 1.2%, (NH4)2SO4 0.6%, MgSO4 0.02%, K2HPO4 0.1%. The optimal conditions for producing glutathione occured at initial pH6.0, broth volume 50%~60% and rotational speed 300 r/min. After 24 hours fermentation, the glutathione produced by Saccharomyces cerevisiae was 90.20 mg/L.

Key words: glutathione, Saccharomyces cerevisiae, biomass, fermentation