食品科学 ›› 2007, Vol. 28 ›› Issue (11): 493-496.

• 分析检测 • 上一篇    下一篇

山茱萸肉和种子脂溶性成分的GC-MS分析

 白成科, 李鹏毛, 王喆之   

  1. 陕西师范大学生命科学学院教育部药用植物资源与天然药物化学重点实验室; 陕西师范大学生命科学学院教育部药用植物资源与天然药物化学重点实验室 陕西西安710062; 陕西西安710062;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Analysis of Fat-soluble Components in Cornus officinalis Flesh and Seeds by GC-MS

 BAI  Cheng-Ke, LI  Peng-Mao, WANG  Zhe-Zhi   

  1. College of Life Sciences, Shaanxi Normal University, Key Laboratory for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Ministry of Education Xi’an 710062, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 采用索氏提取法提取山茱萸肉和种子的脂溶性成分,进行甲酯化处理后,用气相色谱-质谱联用技术(GC-MS)对脂溶性成分进行分析和鉴定,并用面积归一化法测定各成分的相对含量。结果从山茱萸肉中鉴定出31种脂溶性成分,占总检出物的90.91%,脂溶性成分以脂肪酸(61.46%)和烃类化合物(22.03%)为主,其中脂肪酸主要以油酸(18.73%)和亚油酸(27.23%)为主。从山茱萸种子中鉴定了30种脂溶性成分,占总检出物的98.61%,脂肪酸高达97.84%,其中以油酸(58.86%)、硬脂酸(21.00%)、棕榈酸(7.10%)和亚油酸(4.08%)为主,其余非脂肪酸成分含量不到1%。

关键词: 山茱萸, 脂溶性成分, GC-MS

Abstract: The fat-soluble components in the flesh and seeds of Cornus officinalis were extracted by Soxlet extraction, and then esterified and analyzed by gas chromatography-mass spectrometry in the end. The contents of the fatty acids were determined by the normalization method. There are thirty-one and thirty fat-soluble components detected and identified in flesh and seeds of Cornus officinalis, amounting to 90.91% and 98.61% in the total amounts of extractable substances, respectively. The major fat-soluble components in flesh of Cornus officinalis are found to be fatty acids(61.46%) and hydrocarbons(22.03%), with the main fatty acids as 9-Octadecenoic acid (18.73%) and 9, 12-Octadecadienoic acid (27.23%). But the fatty acids in the Cornus officinalis seed are amounting to 97.84%, with the main components as 9-Octadecenoic acid (58.86%), Octadecanoic acid (21.00%), hexadecanoic acid(7.10%), and 9,12-Octadecadienoic acid (4.08%). All non-fatty acid components are under 1%.

Key words: Cornus officinalis, fat-soluble components, GC-MS