食品科学 ›› 2007, Vol. 28 ›› Issue (2): 244-246.

• 分析检测 • 上一篇    下一篇

辣椒制品中苏丹色素的薄层色谱检测方法的研究

 丁长河, 钱林, 任惠华, 李里   

  1.  河南工业大学粮油食品学院; 河南工业大学粮油食品学院 河南郑州450052; 河南郑州450052;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Study on Sudan in Chilli Products by Thin Layer Chromatography

 DING  Chang-He, QIAN  Lin, REN  Hui-Hua, LI  Li   

  1. College of Grain and Food , Henan University of Technology, Zhengzhou 450052, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 本文探索了对辣椒制品进行样品预处理,从中提取添加的苏丹色素,并对其进行薄层色谱法分离鉴定的方法。用石油醚从辣椒粉末中萃取出色素,然后在硅胶薄层色谱板上点样,并在展开剂(石油醚:无水乙醚:甲酸=80:20:1,V/V/V)中展开,进而达到定性检测苏丹I的目的。通过活性白土对辣椒油中苏丹I~IV的吸附及无水乙醇对吸附色素的解吸,分离出苏丹色素,进而在硅胶薄层色谱板上点样,在展开剂(石油醚:无水乙醚:甲酸=80:20:1,V/V/V)中展开,达到定性检测辣椒油中苏丹I~IV的目的。

关键词: 苏丹I~IV, 辣椒制品, 薄层色谱法, 检测

Abstract: A new method to detect Sudan I~IV in hot chilli products was studied in this paper. SudanⅠ could be extracted by ether from chilli powder, and detected by TLC to be spreaded on the solvent of ether/acetic ether/formic acid=80/20/1(V/V/ V) for qualitative assay. Sudan I~IV could be effectively adsorbed by activated clay from tabasco, then desorbed by ethyl alcohol, and finally detected by TLC with the silica gel plate.

Key words: Sudan I~IV, hot chilli products, TLC(thin layer chromatography), detection