食品科学 ›› 2007, Vol. 28 ›› Issue (2): 258-260.

• 分析检测 • 上一篇    下一篇

Folin-Ciocaileu比色法测定蜂花粉中的总酚

 田文礼, 孙丽萍, 董捷, 刘博   

  1. 中国农业科学院蜜蜂研究所; 中国农业科学院蜜蜂研究所 北京100093; 北京100093;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Determination of Total Polyphenolic Compounds in Bee-pollen by Folin-Ciocaileu Method

 TIAN  Wen-Li, SUN  Li-Ping, DONG  Jie, LIU  Bo   

  1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 蜂花粉含有丰富的多酚类化合物,这类化合物具有抗动脉硬化、降低胆固醇、抗氧化和抗辐射等作用,因此可以用来评价蜂花粉的活性。本文采用Folin-Ciocaileu法对蜂花粉中的总酚进行检测。本方法以原儿茶酸为对照品,标准曲线的回归方程为:C=7.9234A-0.0157(R2=0.9999);对稳定性、精密度、重现性、回收率进行了试验,相对标准偏差(RSD)为0.05%~1.43%。该方法简便、快速、准确,是检测蜂花粉中总酚的可靠方法。

关键词: 蜂花粉, 总酚, Folin-Ciocaileu

Abstract: There are rich polyphenolic compounds in bee-pollen, which have multi-functions, such as anti-arteriosclerosis, cholesterol lowering function, anti-oxidation and anti-radiation. Therefore, polyphenolic compounds could be evaluated as the activities of bee-pollen. Total polyphenolic compounds in bee-pollen were determined by Folin-Ciocaileu method. The standard sample is protocatechuic acid, and regressive equation of standard curve is C=7.9234A-0.0157 (R2=0.9999). Furthermore, stability, precision, repetition and recovery rate were assayed with RSD(relative standard deviation) as 0.05%~1.43%. The method is convenient, fast, and exact, it is reliable to determine the total polyphenolic in bee-pollen.

Key words: bee-pollen, total polyphenolic, Folin-Ciocaileu