食品科学 ›› 2007, Vol. 28 ›› Issue (2): 289-292.

• 营养卫生 • 上一篇    下一篇

菜籽肽的制备及其对大鼠血管紧张素Ⅰ转换酶(ACE)活性的影响(英文)

 薛照辉, 尉万聪, 吴谋成, 周志江, 刘春泉   

  1. 天津大学农业与生物工程学院; 中国农业科学院生物技术研究所; 华中农业大学食品科技学院; 江苏省农业科学院 天津300072 江苏省农业科学院; 江苏南京210014; 北京100081; 湖北武汉430070; 天津300072;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Preparation and Effects on Angiotensin I-Converting Enzyme Activity of Rapeseed Peptide(RSP) in Rats

 XUE  Zhao-Hui, WEI  Wan-Cong, WU  Mou-Cheng, ZHOU  Zhi-Jiang, LIU  Chun-Quan   

  1. 1.College of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China; 2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 4.Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 目的:分离菜籽清蛋白水解物并研究混合肽及级分对大鼠血管紧张素Ⅰ转换酶(ACE)活性的影响。方法:采用葡聚糖凝胶过滤色谱(SephadexG-25)分离菜籽肽;离体培养检测血管紧张素Ⅰ转换酶(ACE)活性。结果:菜籽肽(RSP-R)及分离的三个级分(RSP-Ⅰ、Ⅱ和Ⅲ)对高血压大鼠ACE活性均有明显抑制作用,其中级分Ⅲ抑制能力最强。结论:菜籽清蛋白水解多肽具有抑制ACE活性能力,这一结果有助于菜籽清蛋白的广泛应用。

关键词: 菜籽清蛋白, 菜籽肽, 血管紧张素转换酶(ACE)抑制因子, 洛汀新, 生物活性肽

Abstract: Objective :To isolate the Rapeseed Albumin hydrolysate and study the effects on the activity of angiotension Ⅰ- converting enzyme(ACE) of the rapeseed peptides on rats and the grades. Methods: ①Using Sephadex G-25 column with water as eluant; ②Spontaneously hypertensive rats(SHR) were used as animal model, the changes of ACE activity were investigated in vitro. Results :Three purified grades were isolated(RSP-Ⅰ,Ⅱ, and Ⅲ) by Sephadex G-25 column. RSP-R and three fractions exert remarkable inhibition on ACE activity, among which RSP-3 showed the highest inhibition. Conclusion: Proteolysis of rapeseed albumin can result ACE inhibitory activity in peptides. The high quality of RSP can be used directly in the supplementation of foods and can also widen the field of application of rapeseed albumin.

Key words: rapeseed albumin, rapeseed peptide(RSP), angiotensin I-converting enzyme(ACE) inhibitors, lotensin, bio-active peptides