红菇蜡伞子实体水溶性多糖提取工艺研究

食品科学 ›› 2007, Vol. 28 ›› Issue (6): 156-159.

红菇蜡伞子实体水溶性多糖提取工艺研究

陈芝兰, 张涪平, 钟国辉, 熊伟, 田发益, 钟政昌, 杨爽

西藏农牧学院生物中心；西藏农牧学院农学系；西藏农牧学院公共基础部；西藏农牧学院生物中心西藏林芝860000；西藏林芝860000；

出版日期:2007-06-15 发布日期:2011-12-31

Optimization of Conditions for Extracting Crude Polysaccharide from Hygrophorus russula

CHEN Zhi-Lan, ZHANG Fu-Ping, ZHONG Guo-Hui, XIONG Wei, TIAN Fa-Yi, ZHONG Zheng-Chang, YANG Shuang

1.Center of Biology, Tibet Agricultural and Animal Husbandry College, Linzhi 860000, China; 2. Department of Agronomy, Tibet Agricultural and Animal Husbandry College, Linzhi 860000, China; 3. Department of Public Teaching, Tibet Agricultural and Animal Husbandry College, Linzhi 860000, China

Online:2007-06-15 Published:2011-12-31

摘要/Abstract

摘要： 采用单因素考察和正交优化法确立红菇蜡伞多糖提取工艺,结果表明红菇蜡伞干燥子实体萃取粗多糖较佳的条件为:粒度以粉碎60目筛,选用菌盖,提取剂为蒸馏水,选择料水比为1:10(W/V),沉淀剂达到80%的乙醇浓度,萃取时间为4h,萃取温度选择在90℃,提取2次。在此条件下可得到粗多糖3%～4%。正交试验表明影响红菇蜡伞多糖得率的主次因素为乙醇浓度>料水比>提取温度>提取时间,各因素的最佳条件为:时间4h,温度80℃,料水比1:10,乙醇浓度85%。在此条件下多糖得率为6.52%,提取粗多糖含量在49.17%～69.86%,多糖提取量以A3B2C1D3组合最多。

关键词: 红菇蜡伞, 多糖, 提取

Abstract: Single factor tests and orthogonal test design were applied to optimize the extraction process of polysaccharides from Hygrophorus russula. The results showed that the optimal conditions for powdered versus chipped fruit bodies of Hygrophorus russula are particle size 60-mesh sieve , choice of fruit bodies of Hygrophorus russula, distilled water as extraction solvent, solid to liquid ratio 1:10, ethanol concentration 85%, time 4 h, temperature 90 ℃ and two consecutive extractions with crude polysaccharide yield of 3%～4%. The results of orthogonal test showed that polysaccharide yield is affected mainly by the ethanol concentration used, followed by temperature, solid to liquid ratio and treatment time. The optimal conditions are extraction time 4 h, temperature 80 ℃, solid to liquid ratio 1:10, and ethanol concentration 85%. Under these conditions, A3B2C1D3 were the optimal conditions with the highest yield of 6.52% and percentage yield of crude polysaccharide 49.17%～69.86%.

Key words: Hygrophorus russula, polysaccharide, extraction

陈芝兰, 张涪平, 钟国辉, 熊伟, 田发益, 钟政昌, 杨爽. 红菇蜡伞子实体水溶性多糖提取工艺研究[J]. 食品科学, 2007, 28(6): 156-159.

CHEN Zhi-Lan, ZHANG Fu-Ping, ZHONG Guo-Hui, XIONG Wei, TIAN Fa-Yi, ZHONG Zheng-Chang, YANG Shuang. Optimization of Conditions for Extracting Crude Polysaccharide from Hygrophorus russula[J]. FOOD SCIENCE, 2007, 28(6): 156-159.

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