食品科学 ›› 2007, Vol. 28 ›› Issue (6): 240-242.

• 生物工程 • 上一篇    下一篇

酶法提取蛋壳膜中的角蛋白

 杨德玉, 李珍, 高新, 丁兆兰, 刘鹏, 张小燕   

  1. 西北大学化学系; 西安力邦制药有限公司; 西北大学食品工程系; 西安新华科工贸学校; 西北大学食品工程系 陕西西安710069; 陕西西安710075; 陕西西安710069; 陕西西安710077;
  • 出版日期:2007-06-15 发布日期:2011-12-31

Enzyme Extraction of Keratin from Eggshell Membrane

 YANG  De-Yu, LI  Zhen, GAO  Xin, DING  Zhao-Lan, LIU  Peng, ZHANG  Xiao-Yan   

  1. 1.Department of Chemistry, Northwest Univesity, Xi’an 710069, China; 2.Libang Pharmaceutical Factory of Xi’an Co. Ltd., Xi’an 710075, China; 3.Department of Food Science and Technology, Northwest Univesity, Xi’an 710069, China; 4.The Industry of Xinhua Technology Trade School in Xi’an, Xi’an 710077, China
  • Online:2007-06-15 Published:2011-12-31

摘要: 以鸡蛋蛋壳膜为原料,酶法提取蛋壳膜中角蛋白的研究,对酶的用量、提取时间、提取温度、提取的最佳pH值、反应的固液比进行了探讨,在最佳工艺条件下:酶用量为蛋膜量的3%(W/W),固液比1:15,pH9~10,提取温度55~65℃,提取时间60min,角蛋白产率可达到26%。

关键词: 蛋壳膜, 角蛋白, 碱性蛋白酶

Abstract: In this paper, the principle of experiment and operation method of extracting keratin from eggshell membrane with alkaline protease was introduced. From the results, the optimum conditions were obtained, including the quantity of alkaline protease 2%(W/W), solid-liquid ratios 1:15, pH9~10, temperature 55~65 ℃, and reaction time 60 min etc. The keratin yield reaches 26%.

Key words: eggshell membrane, keratin, alkaline protease