食品科学 ›› 2007, Vol. 28 ›› Issue (7): 373-376.

• 分析检测 • 上一篇    下一篇

鸡蛋黄中磷脂的提取及分析

 张佑红, 陈燕, 朱雄伟, 秦琴, 吴元欣, 杜进, 谈雅静, 候畅, 赵亮, 吕中   

  1. 武汉工程大学湖北省新型反应器与绿色化学工艺重点实验室; 北京大学医学部; 武汉化工学院; 武汉工程大学湖北省新型反应器与绿色化学工艺重点实验室 湖北武汉430073; 湖北武汉430073; 北京100083; 湖北武汉430023;
  • 出版日期:2007-07-15 发布日期:2011-10-24

Extraction and Analysis of Phospholipids in Egg Yolk

 ZHANG  You-Hong, CHEN  Yan, ZHU  Xiong-Wei, QIN  Qin, WU  Yuan-Xin, DU  Jin, TAN  Ya-Jing, HOU  Chang, ZHAO  Liang, 吕Zhong   

  1. 1.Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China;2.Medicine School, Beijing University, Beijing 100083, China; 3.Wuhan Institute of Technology, wuhan 430023, China
  • Online:2007-07-15 Published:2011-10-24

摘要: 本研究利用磷脂在丙酮中的相对不溶性,将磷脂从蛋黄中提取出来,用分光光法在630nm下测量蛋黄中磷脂的含量。然后以纸层析为主要的定性检测手段来检测磷脂X基团,用薄层色谱法对磷脂混合物的各个成分进行定性分析。

关键词: 蛋黄磷脂, 提取, 纸层析, 薄层层析

Abstract: Based on relative indissolubility of phospholipid in acetone,phospholipids in egg yolk were extracted in this research. The content of phosphorus was determined with spectrophometry at 630nm. Group X in phospholipid was detected by paper chromatography. At least four fractions of the phospholipids were separated by thin layer chromagraphy and the phenomena was explained.

Key words: lecithin, extract, paper chromatography, thin layer-chromatography