食品科学 ›› 2007, Vol. 28 ›› Issue (9): 199-202.

• 工艺技术 • 上一篇    下一篇

香菇多糖的提取工艺比较

 刘宁, 李健   

  1. 东北林业大学材料科学与工程学院; 哈尔滨商业大学食品工程学院 黑龙江哈尔滨150036哈尔滨商业大学食品工程学院; 黑龙江哈尔滨150076;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Study on Comparison of Extraction Methods of Lentinan

 LIU  Ning, LI  Jian   

  1. 1.College of Material Science and Engineering,Northeast Forestry University,Harbin 150036,China;2.College of Food Sciences and Engineering,Harbin University of Commerce,Harbin 150076,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 本实验以香菇为原料,应用酶解法提取香菇多糖。考察了影响实验的因素即酶用量、酶制剂作用时间、作用温度和pH值对酶解法提取率的影响。并将酶解提取方法与传统提取方法进行分析比较。利用红外光谱和紫外吸收光谱分析浸提物。混合酶解提取法香菇多糖最高提取率为12.90%,比传统水浴浸提法高出6.26%,在时间上仅用了传统方法的1/3。其产品在紫外吸收时,粗产品杂质含量少,比传统浸提方法更接近标准品。研究结果表明,混合酶解法提取香菇多糖的方法优于传统水浴浸提法。

关键词: 香菇, 香菇多糖, 酶解法, 提取

Abstract: In this research,with Lentinus edodes as raw material,the lentinan was extrated by enzymolysis method.The factors affecting extraction such as extraction time,reaction temperature,enzyme dosage and pH value were studied,and enzmolysis method was compared with traditional extrction method.The extraction product analyzed by infrared spectrogram and ultraviolet spectrogram.The highest extraction rate of admixture enzymolysis method is 12.90% and higher 6.26% than that of traditional extraction by heat water.Meanwhile,the impurity content in product is the lowest among the three methods,and the quality approaches to the standard of pure arabinogalactan much more.The results of experiment indicated that the admixture enzymolysis method is superior to traditional extraction by heat water and microscope wave.

Key words: Lentinus edodes, lentinan, enzymatic methods, extraction