食品科学 ›› 2008, Vol. 29 ›› Issue (12): 283-287.

• 工艺技术 • 上一篇    下一篇

响应曲面法优化桔梗多糖提取条件研究(英文)

 阮征, 周泉城, 邓泽元, 印遇龙   

  1. 南昌大学食品科学与技术国家重点实验室,南昌大学生命科学学院食品科学与工程系; 山东理工大学轻工与农业工程学院;
  • 出版日期:2008-12-15 发布日期:2011-12-08

Study on Optimization of Extraction Conditions of Platycodon grandiflorum A. DC Polysaccharide Using Response Surface Methodology

 RUAN  Zheng, ZHOU  Quan-Cheng, DENG  Ze-Yuan, YIN  Yu-Long   

  1. 1. National Laboratory of Food Science and Technology,Department of Food Science and Engineering,College of Life Science,Nanchang University,Nanchang 330031,China;2. School of Light Industry and Agriculture Engineering, Shandong University of Technology,Zibo 255049,China
  • Online:2008-12-15 Published:2011-12-08

摘要: 以桔梗根为实验材料,基于单因素(提取时间、提取温度、物料比、pH值和NaCl浓度)试验结果,确定桔梗多糖提取主要因素,分别为物料比、提取时间和提取温度。通过中心组合试验设计,得到最佳提取条件为物料比34.43,提取时间89.83min,提取温度52.47℃,桔梗多糖提取率为39.87%。通过验证实验,此模型确认有效。

关键词: 响应曲面, 桔梗, 多糖, 提取条件, 优化

Abstract: Platycodon grandiflorum A. DC.(PADC) root was taken as experiment material to extract polysaccharide. On the base of single factor tests(extraction time,extraction temperature,liquid-solid ratio,solvent pH value and NaCl concentration) ,the study concluded the main factors affecting the extraction of PADC polysaccharide,which are liquid-solid ratio,extraction time and extraction temperature. Then through central-composite test design,the extraction conditions were concluded as liquid-solid ratio 34.43,extraction time 89.83 min and extraction temperature 52.47 ℃. By means of validation experiments,the adequacy of this model was confirmed.

Key words: response surface methodology, Platycodon grandiflorum A. DC, polysaccharide, extraction conditions, optimization