食品科学 ›› 2008, Vol. 29 ›› Issue (6): 273-275.

• 生物工程 • 上一篇    下一篇

前体物质与真菌激发子对虫草菌素产量的影响

 李祝, 郁建平, 梁宗琦, 肖洋   

  1. 贵州大学生命科学院真菌资源研究所; 贵州大学生化营养研究所; 贵州省产品质量检验检测院
  • 出版日期:2008-06-15 发布日期:2011-08-26

Effect of Precursor and Fungal Elicitor on Cordycepin Production

 LI  Zhu, YU  Jian-Ping, LIANG  Zong-Qi, XIAO  Yang   

  1. 1.Institute of Fungus Resource, College of Life Science , Guizhou University; 2. Institute of Biochemistry and Nutrition, Guizhou University; 3. Institute of Products Detection and Determination of Guizhou Province
  • Online:2008-06-15 Published:2011-08-26

摘要: 将腺苷作为前体,疫霉Phytophthora sp.(YL)作为真菌激发子,研究它们对蛹虫草(Cordyceps millitaris97-0706)菌株虫草菌素产量及生物量的影响。结果表明:腺苷以3.6mg/ml的量在发酵前(0h)单独加入,摇瓶培养7d,0706菌株的虫草菌素含量达到了8.55±0.09mg/g(p<0.01),是对照的2.2倍。当2.0mg/ml腺苷与80μg/mlYL混合加入,0706菌株的虫草菌素含量为8.25±0.08mg/g,与只用2.0mg/ml腺苷处理的含量(8.21±0.074mg/g)无显著差异(p>0.05),仅表现了腺苷的作用;生物量方面,腺苷和YL的同时加入,起到了协同提高的作用,0706菌株的生物量最终达到46.11±0.10g/L(p<0.01),是对照的1.9倍。

关键词: 腺苷, 虫草菌素, 激发子, 蛹虫草

Abstract: Effects of adenosine and Phytophthora sp. (YL) on the cordycepin production and biomass of Cordyceps millitaris 97-0706 were studied. The ruselts showed that 3.6 mg/ml adenosine added into shake flask culture before fermentation, after 7 d the content of cordycepin reaches to 8.55±0.09 mg/g (p<0.01), 2.2 times more than that of control; 2.0 mg/ml adnosine and 80μg/ml YL together added into shake flask at starting , the cordycepin production of 0706 reaches to 8.25±0.08 mg/g, which is no significant difference (p>0.05) to cordycepin production with only 2.0 mg/ml adenosine added (8.21±0.074 mg/g). But adenosine and YL could cooperate to enhance the mycelia biomass of 0706 (46.11±0.10 g/L), 1.9 times of that of control.

Key words:  , adenosine; cordycepin; elicitor; Cordyceps millitaris;