关键词: 干香菇, 超高效液相色谱-串联质谱法, 多菌灵, 噻菌灵

Abstract:

A method for simultaneous analysis of carbendazim and thiabendazole in dried shiitake was developed using ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). Sample was extracted with 0.02 mol/L hydrochloric acid solution containing 20% methanol, adjusted pH to 6－8, purified with dichloromethane, dried by rotary evaporator, and dissolved in 30% methanol solution. The sample was detected by the UPLC-MS/MS, and quantified by external standard method. Results showed that the linear ranges for determination of carbendazim and thiabendazole by this method were both between 1.0 μg/L and 100.0 μg/L (R2 > 0.99); the average recoveries for the two pesticides in dried shiitake spiked at three levels (1.0, 5.0 and 10.0 μg/kg) were 78%－106%, and the RSDs were 7.9%－9.8% (n = 6); the limit of detection was 1.0 μg/kg; the method could be quickly finished in 3 min and be applied in large amount detection of samples.

Key words: dried mushroom, ultra-performance liquid chromatography - tandem mass spectrometry (UPLC - MS/MS), carbendazim, thiabendazole