食品科学 ›› 2010, Vol. 31 ›› Issue (16): 117-121.doi: 10.7506/spkx1002-6630-201016025

• 工艺技术 • 上一篇    下一篇

响应面法优化超声辅助提取浒苔多糖的工艺

郭 雷1,2,陈 宇1   

  1. 1.淮海工学院 江苏省海洋生物技术重点实验室 2.江苏省海洋资源开发研究院
  • 收稿日期:2010-01-27 修回日期:2010-07-08 出版日期:2010-08-25 发布日期:2010-12-29
  • 通讯作者: 郭雷 E-mail:leiguoo@sina.com
  • 基金资助:

    江苏省自然科学基金项目(BK2009631);江苏省海洋资源开发研究院基金项目(HK09004)

Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Enteromorpha prolifera by Response Surface Methodology

GUO Lei1,2,CHEN Yu1   

  1. 1. Key Laboratory of Marine Biotechnology of Jiangsu Province, Huaihai Institute of Technology, Lianyungang 222005, China;
    2. Jiangsu Marine Resources Development Reasearch Institute, Lianyungang 222004, China
  • Received:2010-01-27 Revised:2010-07-08 Online:2010-08-25 Published:2010-12-29
  • Contact: GUO Lei1 E-mail:leiguoo@sina.com

摘要:

为优化浒苔多糖的提取工艺条件,采用超声辅助提取技术提取浒苔多糖,考察3 个变量(超声温度、超声时间和液料比)对浒苔多糖收率的影响,并通过响应面设计法确定浒苔多糖超声辅助提取技术的最佳工艺条件。结果表明:最佳工艺条件为超声温度80℃、超声时间28min、液料比63:1(mL/g),按此工艺条件提取浒苔多糖,收率为25.84mg/g;验证实验表明,实际浒苔多糖收率与模型预测值相近。采用响应面法优化浒苔多糖超声辅助提取工艺可行。

关键词: 浒苔, 多糖, 超声辅助提取, 响应面法, 工艺优化

Abstract:

We report the use of response surface methodology for the optimization of process conditions for the ultrasonicassisted hot water extraction of polysaccharides from Enteromorpha prolifera. The effects of three independent variables (namely, extraction temperature, length of extraction and ratio of water to material) on the yield of polysaccharides from E. prolifera (PEP) were evaluated. Extraction temperature of 80 ℃, length of extraction of 28 min and ratio of water to material of 63:1 (mL/g) were found optimum. Under these optimized conditions, the experimental value of PEP yield was 25.84 mg/g, in good agreement with the predicted value. Therefore, the optimized process is very reliable.

Key words: Enteromorpha prolifera, polysaccharide, ultrasonic-assisted extraction, response surface methodology, technique optimization

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