关键词: 虎杖, 白藜芦醇, 白藜芦醇苷, 发酵

Abstract:

Objective: To explore the fermentation and extraction processing of resveratrol from Polygonum cuspidatum. Methods: Polygonum cuspidatum from Hanzhong area was used as the material to explore the optimal fermentation and extraction conditions of resveratrol by orthogonal experiments. Results: The optimal conditions for fermentation were fermentation temperature of 45 ℃, material-liquid ratio of 1:3, and fermentation time of 48 h. The conversion rate of polydatin was 96.1% under the optimal fermentation conditions. The optimal conditions for the extraction of resveratrol were 98% methanol as solvent, solid-liquid ratio of 1:6, extraction time of 1 h, and extraction repeat number of 3. The yield of resveratrol was 1.912% under the optimal extraction conditions. The extraction rate of resveratrol could reach up to 90% through optimal fermentation and extraction. Conclusion: These studies provide a theoretical reference for the fermentation and extraction of resveratrol from Polygonum cuspidatum.

Key words: Polygonum cuspidatum Sieb.et.Zucc, resveratrol, polydatin, fermentation