食品科学 ›› 2011, Vol. 32 ›› Issue (12): 160-163.doi: 10.7506/spkx1002-6630-201112035

• 工艺技术 • 上一篇    下一篇

柿皮果胶纤维素酶法提取工艺

张 娜,陈锦屏*,严 静,李仁杰   

  1. 陕西师范大学食品工程与营养科学学院
  • 出版日期:2011-06-25 发布日期:2011-06-10

Optimizing Enzymatic Extraction of Pectin from Persimmon Peel

ZHANG Na,CHEN Jin-ping*,YAN Jing,LI Ren-jie   

  1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 采用纤维素酶法辅助提取柿皮中的果胶,探讨提取过程中各因素对果胶提取率的影响。结果表明:柿皮果胶纤维素酶法提取的最佳工艺条件为提取温度45℃、加酶量3.0mg/g、提取液pH4.0、料液比1:35、提取时间2h,测得果胶的提取率为8.87%。该方法简便、快捷,可用于柿皮果胶的提取和测定。

关键词: 柿皮, 果胶, 纤维素酶, 提取工艺

Abstract: The extraction of pectin from persimmon peel with the aid of cellulase was optimized by orthogonal array design on the basis of one-factor-at-at-a-time. The optimum extraction parameters were identified as follows: extraction temperature of 45 ℃, enzyme dosage of 3.0 mg/g, pH 4.0, material-to-liquid ratio of 1:35 and extraction time of 2 h, and the resulting extraction rate of pectin was 8.87%. This method has the advantages of simplicity and convenience and can therefore be used for the extraction and determination of pectin.

Key words: persimmon peel, pectin, cellulase, extraction process

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