乳源酪蛋白糖巨肽抗细胞凋亡干预小鼠溃疡性结肠炎效应研究

摘要/Abstract

摘要： 研究乳源酪蛋白糖巨肽(CGMP)对溃疡性结肠炎小鼠结肠黏膜细胞凋亡的影响。利用恶唑酮诱导小鼠溃疡性结肠炎(UC)模型，采用低、中、高剂量的CGMP组(剂量分别为5、50、500mg/(kg·d))和药物柳氮磺胺吡啶(剂量为40mg/(kg·d))连续灌胃4d，正常对照组和模型对照组每天灌胃相应剂量的超纯水；用HE染色和TUNEL法检测小鼠结肠大体形态损伤和病理组织学及其细胞凋亡变化。结果表明：低、中、高剂量CGMP组均可在一定程度上改善恶唑酮诱导的结肠炎中结肠病理损伤和细胞凋亡表现，尤其以中剂量CGMP组的作用较为明显，与药物治疗组相比不存在显著性差异。因而，证实CGMP作为一种生物活性物质可以通过抗结肠组织细胞凋亡具有抑制溃疡性结肠炎的功能。

关键词: 酪蛋白糖巨肽, 细胞凋亡, 小鼠, 恶唑酮, 溃疡性结肠炎

Abstract: In order to explore the effect of casein glycomacropeptide (CGMP) on the apoptosis of colonic mucosal cells in mice with oxazolone-induced ulcerative colitis, the mice were treated respectively with CGMP at the doses of 5, 50 mg/(kg·d) and 500 mg/(kg·d) and sulfasalazine at the dose of 40 mg/(kg·d) by gavage for 4 consecutive days to establish high-, medium- and low-dose CGMP groups and positive control group. The same dose of ultra-pure water was once daily given to the normal control and model control groups. HE staining and TdT2-mediated dUTP nick end labeling (TUNEL) assay were used to evaluate morphological and histological damage as well as epithelial cell apoptosis in the colon of the mice with oxazolone-induced ulcerative colitis. The results indicated that CGMP at each doses revealed obvious improvement on oxazolone-induced ulcerative colitis, and the improving effect at the dose of 50 mg/(kg·d) did not significantly differ from that of sulfasalazine. In conclusion, CGMP, a bioactive substance, has promising potential as a nutritional therapy for ulcerative colitis through apoptosis.

Key words: casein glycomacropeptide, apoptosis, mice, oxazolone, ulcerative colitis

中图分类号:

TS201.2

王华，陈庆森*. 乳源酪蛋白糖巨肽抗细胞凋亡干预小鼠溃疡性结肠炎效应研究[J]. 食品科学, 2012, 33(1): 230-234.

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