基质固相分散-气相色谱检测菊花中有机磷农药残留

食品科学 ›› 2012, Vol. 33 ›› Issue (18): 216-219.

基质固相分散-气相色谱检测菊花中有机磷农药残留

沈丹玉1，汤富彬1,*，袁新跃2，钟冬莲1，莫润宏1

1.中国林业科学研究院亚热带林业研究所，国家林业局经济林产品质量检验检测中心 2.富阳市质量计量监测中心

收稿日期:2011-07-15 修回日期:2012-07-23 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: 汤富彬 E-mail:tfb22@163.com
基金资助:
中央级公益性科研院所基本科研业务费专项资金

Determination of Organophosphorus Pesticide Residues in Flos Chrysanthemi by Matrix Solid Phase Dispersion-Gas Chromatography

Received:2011-07-15 Revised:2012-07-23 Online:2012-09-25 Published:2012-09-07

摘要/Abstract

摘要： 建立菊花中14种有机磷农药残留量的气相色谱分析方法。样品用乙腈-丙酮(4:1，V/V)提取，经基质固相分散技术净化，以乙腈-甲苯(3:1，V/V)洗脱，气相色谱-火焰光度检测，外标法定量。在0.05～1.0mg/kg的添加水平，14种有机磷的平均回收率在84.54%～108.54%之间，相对标准偏差在0.66%～6.31%，该方法的检出限为0.01～0.04mg/kg。该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求。采用所建立方法测定了黄山贡菊、杭白菊和胎菊3种代表性菊花中14种有机磷农药残留量，结果表明，黄山贡菊检出乐果1.68mg/kg、毒死蜱0.17mg/kg、硫环磷0.28mg/kg，杭白菊检出毒死蜱0.09mg/kg，胎菊检出乐果0.25mg/kg、毒死蜱0.14mg/kg、伏杀磷0.08mg/kg。

关键词: 菊花, 基质固相分散, 气相色谱, 有机磷农药

Abstract: An analytical method was developed for the simultaneous determination of 14 organophosphorus pesticides in Flos Chrysanthemi by using matrix solid phase dispersion (MSPD) and gas chromatography (GC). Flos Chrysanthemi samples were extracted with acetonitrile-acetone (4:1, V/V) and cleaned up by matrix solid phase dispersion (MSPD), eluted with acetonitrile-toluene (3:1, V/V) and determined by GC with a flame photometric detector. The recovery rates of 14 organophosphorus pesticides were 84.54%～108.54% with relative standard deviation of 0.66%－6.31% at spiked levels of 0.05－1.0 mg/kg. The limit of detection was 0.01－0.04 mg/kg. The proposed method was characterized by simplicity, higher sensitivity and accuracy. The contents of dimethoate, chlorpyrifos and phosfolan in Huangshan Gongju chrysanthemum were 1.68, 0.17 mg/kg and 0.28 mg/kg, respectively, the content of chlorpyrifos in Hangbaiju chrysanthemum was 0.09 mg/kg, and the contents of dimethoate, chlorpyrifos and phosalone in Hangbaiju chrysanthemum bud were 0.25, 0.14 mg/kg and 0.08 mg/kg, respectively as determined by this method.

Key words: Chrysanthemum, organophosphorus pesticides, matrix solid phase dispersion (MSPD), gas chromatography (GC)

中图分类号:

沈丹玉1，汤富彬1,*，袁新跃2，钟冬莲1，莫润宏1. 基质固相分散-气相色谱检测菊花中有机磷农药残留[J]. 食品科学, 2012, 33(18): 216-219.

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