食品科学 ›› 2012, Vol. 33 ›› Issue (9): 247-250.doi: 10.7506/spkx1002-6630-201209052

• 营养卫生 • 上一篇    下一篇

蜂胶对肝癌细胞增殖及其脂肪沉积的影响

田文礼,赵亚周,彭文君*   

  1. 中国农业科学院蜜蜂研究所,农业部授粉昆虫生物学重点开放实验室,国家蜂产品加工技术研发中心
  • 出版日期:2012-05-15 发布日期:2012-05-07
  • 基金资助:
    国家现代农业(蜜蜂)产业技术体系项目(NYCYTI-43-KXJ17);科研院所技术开发研究专项(2009EG134274)

Effect of Propolis on Liver Cancer Cell Proliferation and Fat Deposition

TIAN Wen-li,ZHAO Ya-zhou,PENG Wen-jun*   

  1. (Key Laboratory for Insect-Pollinator Biology, Ministry of Agriculture, National R&D Center for Bee Product Processing, Institute of Apiculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China)
  • Online:2012-05-15 Published:2012-05-07

摘要: 为评价蜂胶对人体肝癌细胞增殖能力和细胞中脂肪沉积程度的影响,以期对蜂胶控制肝细胞病变的效果进行初步量化。采用液质联用仪测定蜂胶中的总黄酮含量达到(191.472±1.986)mg/g;通过凋亡细胞琥珀酸脱氢酶检测法对处理后的肝癌细胞增殖水平进行测定,发现tg9处理组(原蜂胶液+橄榄油(体积比1:2),给药量200μg/mL)的吸光度最低,为0.647,说明抑制细胞增殖的水平较高;利用油酸诱导法检测蜂胶样品处理后的肝癌细胞中甘油三酯含量为410.333~459.672nmol/L,未能达到控制细胞中脂肪沉积的效果。提示蜂胶样品对脂肪肝的预防效果欠佳,而对肝癌细胞的增殖现象具有较明显的抑制作用。

关键词: 蜂胶, 肝癌细胞, 增殖, 脂肪沉积

Abstract: The objective of this study is to preliminarily quantify the control efficacy of human liver cancer by propolis through evaluating the inhibitory effect on HCC cell proliferation and fat deposition. The content of flavonoids in propolis was determined by HLPC-MS to be (191.472±1.986) mg/g. The proliferation of proplis-treated hepatoma cells was evaluated by MTT. The tg9 group (propolis:olive oil =1:2, V/V) revealed the strongest inhibitory effect on cell proliferation. The triglyceride (TG) contents in all treatment groups were in the range of 410.333-459.672 nmol/L. Therefore, propolis can not control fat deposition in cells and the preventative effect of propolis on fatty liver is not satisfactory. However, it has strong inhibitory effect on the proliferation of liver cancer cells.

Key words: propolis, hepatoma cells, proliferation, fat deposition

中图分类号: