食品科学 ›› 2013, Vol. 34 ›› Issue (11): 67-71.doi: 10.7506/spkx1002-6630-201311016

• 基础研究 • 上一篇    下一篇

紫叶稠李果实花色苷的抗氧化活性

冷 梅,刘 荣   

  1. 东北林业大学林学院,黑龙江 哈尔滨 150040
  • 收稿日期:2012-03-30 修回日期:2013-04-28 出版日期:2013-06-15 发布日期:2013-06-03
  • 通讯作者: 冷梅 E-mail:lengmeidx@126.com
  • 基金资助:

    中央高校基本科研业务费专项(DL12CA11)

Antioxidant Activity of Padus virginiana Anthocyanins

LENG Mei,LIU Rong   

  1. College of Forestry, Northeast Forestry University, Harbin 150040, China
  • Received:2012-03-30 Revised:2013-04-28 Online:2013-06-15 Published:2013-06-03
  • Contact: LENG Mei E-mail:lengmeidx@126.com

摘要:

目的:研究紫叶稠李果实花色苷的体外抗氧化活性和作用于肿瘤细胞后,细胞内抗氧化酶的活性和氧化产物的含量。方法:采用分光光度法、水杨酸比色法、邻苯三酚自氧化法、硫代硫酸钠滴定法、铁氰化钾还原法测定对DPPH自由基、·OH、O2-·、H2O2的清除能力以及总还原能力,采用试剂盒法测定肿瘤细胞内GSH-Px、CAT、T-SOD活性和MDA含量。结果:紫叶稠李花色苷清除4种自由基的能力和总还原能力都小于VC,并且作用于肿瘤细胞后,细胞内GSH-Px、CAT、T-SOD的活性都随紫叶稠李花色苷质量浓度的升高而减少,具有一定的量效关系,而细胞内MDA的含量随紫叶稠李花色苷质量浓度的升高而增加,呈线性关系。结论:紫叶稠李花色苷的体外抗氧化活性较强,并对肿瘤细胞内抗氧化酶的活性和氧化产物的含量具有一定影响。

关键词: 紫叶稠李, 花色苷, HT29细胞, HepG2细胞, HeLa细胞

Abstract:

Objective: To explore the in vitro antioxidant activity of Padus virginiana fruit anthocyanins and determine
the activity of intracellular antioxidant enzymes and the content of oxidative products. Methods: The scavenging activity
against DPPH, superoxide anion, hydroxyl free radicals and hydrogen peroxide and total reducing power were determined
by spectrophotometry, salicylic acid colorimetric method, pyrogallol self-oxidation, sodium thiosulfate titration and
potassium ferricyanide reduction method, respectively. The activities of GSH-Px, CAT and T-SOD and MDA content in
tumor cells were determined. Results: The anthocyanins extracted from Padus virginiana fruits had weaker scavenging
effects on the free radicals and hydrogen peroxide and lower total reducing power than VC. The activities of the antioxidant
enzymes GSH-Px, CAT, T-SOD declined when exposed to increasing of these anthocyanins, indicating the existence of a
concentration-dependen relationship. Moreover, intracellular MDA content linearly increased as the concentration of Padus
virginiana anthocyanins increased. Conclusion: Padus virginiana anthocyanins have strong antioxidant capacity and obvious
impact on the activity of antioxidant enzymes and the contents of oxidative products in tumor cells.

Key words: Padus virginiana, anthocyanins, HT29 cells, HepG2 cells, HeLa cells

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