食品科学

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TEMPO/STA法与Baird-Parker平板计数法测定食品中金黄色葡萄球菌的比较研究

凌秀梅,陈礼玲,张 敏,吴远根,叶 梅,万渝平     

  1. 1.成都市产品质量监督检验院,四川 成都 610041;2.上海交通大学环境科学与工程学院,上海 200240
  • 出版日期:2013-10-25 发布日期:2013-09-28

Reliability Comparison of TEMPO/STA Method with Baird-Parker Method for the Determination of Staphylococcus aureus in Foods

LING Xiu-mei,CHEN Li-ling,ZHANG Min,WU Yuan-gen,YE Mei,WAN Yu-ping   

  1. 1. Chengdu Product Quality Supervision and Inspection Institute, Chengdu 610041, China;
    2. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

使用TEMPO/STA法和Baird-Parker平板计数法对速冻食品、生鲜乳、肉制品、冷食菜和调味品5类共150组样品进行金黄色葡萄球菌加标实验,结果表明两种方法检测结果符合率高达94.67%,并且检测结果P值均大于0.05,无显著性差异。同时使用TEMPO/STA法和Baird-Parker平板计数法对冷食菜、调味品、糕点、方便食品、生鲜乳、速冻食品和生肉7类共300组自然样品进行检测验证,结果显示两种方法的金黄色葡萄球菌检出率分别为33.33%和31.33%,对阳性结果进行统计学分析发现两种方法无显著性差异。

关键词: TEMPO/STA法, Baird-Parker平板计数法, 金黄色葡萄球菌, 食品

Abstract:

In this study, TEMPO/STA method and Baird-Parker method in the Chinese national standard GB
4789.10—2010 were used to count Staphylococcus aureus in 150 samples from five kinds of foods including quickfrozen
foods, raw milk, meat products, cold fish products and condiments. The results showed that there were good
coincidence rate as high as 94.67% and the P value was larger than 0.05, suggesting that no significant difference
between the two methods. The positive rates of Staphylococcus aureus with TEMPO/STA method and Baird-Parker
method were 33.33% and 31.33%, respectively in 300 natural samples assigned to 7 groups including cold dish, condiment,
pastry, convenient foods, raw milk, frozen foods and raw meat. Statistical analysis showed no significant difference
between the two methods.

Key words: TEMPO/STA method, Baird-Parker method, Staphylococcus aureus, food