响应面法优化以菊芋全粉为碳源的双歧杆菌增殖培养条件

doi:10.7506/spkx1002-6630-201323053

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 261-265.doi: 10.7506/spkx1002-6630-201323053

响应面法优化以菊芋全粉为碳源的双歧杆菌增殖培养条件

于济洋1,2，李新华1,*，王杕3，张明2,4，秦宁1，郑凤娥2，王琳1

1.沈阳农业大学食品学院，辽宁沈阳 110866；2.沈阳产品质量监督检验院食品安全所，辽宁沈阳 110022；
3.中国农业科学院农产品加工研究所，北京 110193；4.辽宁大学生命科学院，辽宁沈阳 110036

收稿日期:2013-07-25 修回日期:2013-11-06 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 李新华 E-mail:418147876@qq.com

Optimization of Culture Conditions for Bifidobacterial Enrichment with Whole Powder of Jerusalem artichoke Tubers as Carbon Source by Response Surface Analysis

YU Ji-yang1,2，LI Xin-hua1,*，WANG Di3，ZHANG Ming2,4，QIN Ning1，ZHENG Feng-e2，WANG Lin1

1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China；
2. Food Safety Institute, Shenyang Product Quality Supervision and Inspection Institute, Shenyang 110022, China；
3. Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences，Beijing 110193, China；
4. School of Life Science, Liaoning University, Shenyang 110036, China

Received:2013-07-25 Revised:2013-11-06 Online:2013-12-15 Published:2014-01-03
Contact: LI Xin-hua E-mail:418147876@qq.com

摘要/Abstract

摘要：

以菊芋全粉、菊粉和葡萄糖作为培养基碳源，研究其对双歧杆菌生长情况的影响，并研究菊芋全粉的发酵性能，运用Design-Expert 8.0设计实验并以响应面分析的方法进行发酵条件优化。结果表明：菊芋全粉、菊粉作为单一碳源对双歧杆菌具有增殖作用，利用菊芋全粉对双歧杆菌的增殖作用要优于菊粉和葡萄糖，双歧杆菌的增殖数量分别为菊粉的5.3倍、葡萄糖的16.8倍，菊芋全粉作为双歧杆菌培养基的碳源的最优发酵条件为：菊芋全粉添加量21g/L、温度36℃、接种量10%。

关键词: 菊芋全粉, 双歧杆菌, 优化, 响应面法

Abstract:

The best carbon source for bifidobacterial growth was selected from inulin, glucose and whole powder of
Jerusalem artichoke Tubers, and the bifidobacterial enrichment culture conditions based on the selected carbon source
were optimized by experimental design using Design-Expert 8.0 and response surface analysis. Both inulin and glucose
were good bifidobacterial growth promoters when used as sole carbon sources；however, whole powder of Jerusalem
artichoke Tubers was more effective in promoting bifidobacterial growth, providing a 5.3-fold and 16.8-fold increase in
bifidobacterial populations after enrichment culture compared to inulin and glucose, respectively. When whole powder of
Jerusalem artichoke Tubers was used as the carbon source, the optimal culture conditions were 36 ℃, 21 g/L of carbon
source concentration and 10% of inoculum concentration.

Key words: whole powder of Jerusalem artichoke Tubers, Bifidobacterium, optimization, response surface analysis

中图分类号:

TQ920.1

于济洋1,2，李新华1,*，王杕3，张明2,4，秦宁1，郑凤娥2，王琳1. 响应面法优化以菊芋全粉为碳源的双歧杆菌增殖培养条件[J]. 食品科学, 2013, 34(23): 261-265.

YU Ji-yang1,2，LI Xin-hua1,*，WANG Di3，ZHANG Ming2,4，QIN Ning1，ZHENG Feng-e2，WANG Lin1. Optimization of Culture Conditions for Bifidobacterial Enrichment with Whole Powder of Jerusalem artichoke Tubers as Carbon Source by Response Surface Analysis[J]. FOOD SCIENCE, 2013, 34(23): 261-265.

参考文献

[1]WU Qianqian, YOU H J, AHN H J, et al.Changes in growth and survival of Bifidobacterium by coculture with Propionibacterium in soy milk,cow’s milk,and modified MRS medium[J].International Journal of Food Microbiology, 2012, 157(1):65-72

[2]李俊洁, 陈庆森.双歧杆菌调理和改善肠道相关疾病作用的研究进展[J].食品科学, 2011, 32(23):326-332

[3]冯大伟, 衣悦涛; 刘广洋等.一种菊芋全粉的制备方法 [P]. 中国专利: CN103005326A.2013-04-03

[4]田向东, 田伟民, 陈志刚.菊芋全粉的生产方法[P]. 中国专利: CN101940306A. 2011-01-12

[5]苗晓洁, 董文宾, 代春吉等.菊糖的性质功能及其在食品工业中的应用[J].食品科技, 2006, (4):9-11

[6]OLIVEIRA R, PEREGO P, OLIVEIRA M, et al.Growth,organic acids profile and sugar metabolism of Bifidobacterium lactis in co- culture with Streptococcus thermophilus: the inulin effect[J].Food Research International, 2012, 48(1):21-27

[7]韩雪, 张兰威.双歧杆菌增殖因子的筛选及培养基的优化[J].食品与生物技术学报, 2005, 24(4):69-72

[8]PRASANNA P, GRANDISON A, CHARALAMPOPOULOS D.Effect of dairy-based protein sources and temperature on growth,acidification and exopolysaccharide production of Bifidobacterium strains in skim milk[J].Food Research International, 2011, 47(1):6-12

[9]KimY, FaqihMN, WangS.Factors affecting gel formation of inulin[J]., 2001, 46(2):135-145

[10]RAHMAN M, KIM W, ITO T, et al.Growth promotion and cell binding ability of bovine lactoferrin to Bifidobacterium longum[J].Anaerobe, 2009, 15(4):133-137

[11]张建平, 张泽生, 王金菊.菊糖及其酶解产物对长双歧杆菌的促生长作用[J].天津科技大学学报, 2011, 26(4):17-20

[12]许威, 罗登林, 陈瑞红.菊粉酸降解动力学研究[[J].食品科学, 2012, 33(15):95-98

[13]郑晓丽, 芦春明, 韩璐.膜分级菊粉及其部分理化性质[J].大连工业大学学报,, 2011, 30(5):337-340

[14]程丹丹, 葛菁萍, 宋刚等.应用响应面法优化乳酸乳球菌DU101发酵培养基[J].东北农业大学学报, 2010, 41(2):116-122

[15]NINESS K.Breakfast foods and the health benefits of inulin and oligofructose[J].Cereal foods world, 1999, 44(2):79-81

[16]孙鹏, 赵旭博, 孙先峰.响应面法优化长双歧杆菌增殖培养基[J].食品科学, 2013, 34(5):207-212

[17]CATIA M, CIDALIA P, ADRIAN H, et al.Review on fermented plant materials as carriers and sources of potentially probiotic lactic acid bacteria e with an emphasis on table olives[J].Trends in Food Science & Technology, 2012, 26(1):31-42

[18]朱俊晨, 李世敏, 魏小青.双歧杆菌对人体的生理作用及研究进展[J].中国微生态学杂志, 2000, 12(l):53-5

[19]陈晓明, 朱太海.菊糖的功能及其在食品工业中的应用[J].食品科技, 2000, (5):34-35

响应面法优化以菊芋全粉为碳源的双歧杆菌增殖培养条件

Optimization of Culture Conditions for Bifidobacterial Enrichment with Whole Powder of Jerusalem artichoke Tubers as Carbon Source by Response Surface Analysis

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