响应面法优化超临界CO2萃取玉米蛋白粉中叶黄素工艺

食品科学 ›› 2013, Vol. 34 ›› Issue (4): 37-41.

响应面法优化超临界CO2萃取玉米蛋白粉中叶黄素工艺

佟万兵¹,曹龙奎²,宋亮¹

1. 黑龙江八一农垦大学
2. 黑龙江八一农垦大学食品学院;黑龙江省农产品加工工程技术研究中心

收稿日期:2011-10-13 修回日期:2012-12-26 出版日期:2013-02-25 发布日期:2013-01-29
通讯作者: 曹龙奎 E-mail:longkuicao@yahoo.com.cn
基金资助:
国家科技部(2009GB2B200100)

Optimizing the process conditions for lutein extraction from corn protein powder by Supercritical CO2

Received:2011-10-13 Revised:2012-12-26 Online:2013-02-25 Published:2013-01-29

摘要/Abstract

摘要： 为获得纯度较高的叶黄素，以玉米蛋白粉为原料，对玉米叶黄素的超临界CO2萃取工艺条件进行研究，采用高效液相色谱法（HPLC）测定叶黄素纯度。用响应面分析法优化超临界CO2萃取叶黄素的工艺参数，在单因素试验基础上选取萃取时间、萃取温度、萃取压力三个主要因素，以叶黄素纯度为响应值，对其提取工艺参数进行优化，得出了最优提取工艺条件为：萃取时间2.3h，萃取温度42℃，萃取压力30Mpa,夹带剂为90%乙醇，添加量5%。在该条件下由响应面模型预测的玉米叶黄素纯度为46.8%，验证值为46.5%。

Abstract: This paper in order to obtain high purity lutein, with corn protein powder as raw materials,to study the extraction process conditions for lutein by supercritical CO2,taking the HPLC as the determination method. The technology parameter of lutein extraction was studied by response surface analytic.Based on the single factor experiment,selecting extraction time,temperature and pressure three main factors,with the purity of lutein as response value to optimize the extraction process parameters and gain the optimum extraction process conditions:extraction time is 2.3h,temperature is 42℃,pressure is 30 MPa,φ(ethanol)=90% as the entrainer and the additive amount is 5%,by which the lutein purity is 46.8% at these conditions and the verification is 46.5%.

中图分类号:

TQ423

佟万兵曹龙奎宋亮. 响应面法优化超临界CO2萃取玉米蛋白粉中叶黄素工艺[J]. 食品科学, 2013, 34(4): 37-41.

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