关键词: 保健酒, 硅胶柱层析法, 气相色谱-质谱联用仪, 乳酸丙酯, 挥发性成分

Abstract:

Volatile compounds of 11 kinds of health wines were analyzed by silica gel column chromatography (SGCC) and
gas chromatography-mass spectrometry (GC-MS). The identification and quantification were carried out by comparing with
standards and searching the NIST 11 Library. The results showed a total of 37 compounds were identified in 11 wine samples,
consisting of 6 alcohols, 12 hydrocarbons, 9 esters, 4 aldehydes, 2 acids, 2 ketones, eugenol and propyl lactate. Among these
compounds, n-propanol, 2-methyl-1-propanol, 3-methyl-1-butanol, ethyl lactate, triethyl citrate, 5-hydroxymethyfurfural
(5-HMF) and acetic acid were found to be prominent components. Methyl lactate was used as an internal standard
to quantify propyl lactate. In these samples, the concentrations of propyl lactate ranged from 0.02 to 0.74 mg/L.
The regression equation for propyl lactate demonstrated good linearity (R2 = 0.995). The limits of detection (LOD) was
0.005 mg/L and the limit of quantitation (LOQ) was 0.01 mg/L. Pareto analysis and one-way analysis of variance (ANOVA)
were carried out to qualitatively analyze the important volatile compounds and differences in the percentage compositions of
alcohols, hydrocarbons, esters and aldehydes in Chinese Jinjiu, Yangchun Zibujiu, Tuopai Goji, Yedao Haiwang, Zhuyeqing
and Zhangyu Sanbian wines by MATLAB 7.0. The ANOVA results showed that a significant difference in hydrocarbons
was detected. The important volatile compounds included 3-methyl-1-butanol, 1-propanol, 2-methyl-1-propanol, 2-butanol,
5-HMF, eugenol, ethyl lactate, ethyl hexanoate, isoamyl acetate, phenethyl acetate, ethyl caprate, triethyl citrate, furfural,
cinnamyl aldehyde, acetic acid, styrene, tetradecane, pentadecane, cetane, 1,4-dimethyl benzene, and diphenylamine.

Key words: heath wine, silica gel column chromatography, gas chromatography-mass spectrometry (GC-MS), propyl lactate, volatile compound