食品科学 ›› 2019, Vol. 40 ›› Issue (1): 216-225.doi: 10.7506/spkx1002-6630-20180531-447

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基于iTRAQ技术研究采后1-甲基环丙烯和乙烯利处理对茭白线粒体蛋白质组变化的影响

罗海波1,周 涛1,孔晓雪1,陶明煊1,姜 丽2,王利斌3,王韦华2,郁志芳2,*   

  1. 1.南京师范大学金陵女子学院,江苏 南京 210097;2.南京农业大学食品科技学院,江苏 南京 210095;3.南京农业大学园艺学院,江苏 南京 210095
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401612);浙江省自然科学基金项目(LY14C200005)

Effect of 1-Methyleyelopropene and Ethylene on Mitochondrial Proteome Changes in Postharvest Zizania latifolia Analyzed Using iTRAQ Labeling Technique

LUO Haibo1, ZHOU Tao1, KONG Xiaoxue1, TAO Mingxuan1, JIANG Li2, WANG Libin3, WANG Weihua2, YU Zhifang2,*   

  1. 1. Ginling College, Nanjing Normal University, Nanjing 210097, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 3. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 为探讨茭白采后衰老的分子机制,应用同位素标记相对与绝对定量蛋白质组学技术研究了茭白常温贮藏期间线粒体蛋白质表达谱的变化及1-甲基环丙烯(1-methyleyelopropene,1-MCP)和乙烯利(ethylene,ET)处理对茭白线粒体蛋白质组变化的影响。结果表明:共鉴定到肽段数大于等于2的可信蛋白1 908 个,与贮藏0 d相比,对照(CK)组、ET和1-MCP处理组茭白贮藏3 d和6 d后,共有315 个蛋白表达量变化倍数在2.0 倍以上且重复组数据统计学差异显著(P<0.05)。生物信息学分析显示代谢途径、次生代谢产物生物合成、氨基酸生物合成与代谢、核苷酸代谢、含碱基小分子代谢途径等可能与茭白采后衰老有关,三羧酸循环、氧化磷酸化、磷酸戊糖途径、C5支链二元酸代谢及氨基酸代谢途径可能在茭白采后衰老中发挥重要作用。这些差异表达蛋白的生物学功能分析表明,茭白采后碳水化合物水解加速,磷酸戊糖途径加强而糖酵解途径和氧化磷酸化减弱,导致能量合成减少,同时形成氧化胁迫,这可能激活Ca2+/MAPKs、细胞色素c和茉莉酸等信号途径,造成初级代谢紊乱和次级代谢产物(如木质素)积累,从而促进细胞凋亡或细胞坏死,最终加速衰老。

关键词: 茭白, 线粒体, 同位素标记相对与绝对定量, 生物信息学, 衰老

Abstract: In order to explore the molecular mechanism of postharvest senescence of Zizania latifolia, the effect of 1-methyleyelopropene (1-MCP) and ethylene (ET) treatments on mitochondrial proteome changes in postharvest Z. latifolia after storage at 25 ℃ for 0 (control), 3 and 6 days were investigated by using isobaric tags for relative and absolute quantification (iTRAQ) labeling. The results showed that a total of 1 908 proteins were identified with at least two peptides, of which 315 proteins showed an 2.0-fold change in their relative quantitation in the untreated, ET-treated, 1-MCPtreated groups at day 3 and 6 of storage as compared to their counterparts at day 0, with statistically significant differences being observed between results from replicate experiments (P < 0.05). A bioinformatics analysis of these differentially expressed proteins revealed that metabolic pathways, the biosynthesis of secondary metabolites, the biosynthesis and metabolism of amino acids, nucleotide metabolism, and the metabolic pathways of nucleobase-containing small molecules were related to postharvest senescence of Z. latifolia and that the citrate cycle, oxidative phosphorylation (OXPHOS), the pentose phosphate pathway (PPP), C5-branched dibasic acid metabolism and amino acid metabolism may play critical roles in postharvest senescence of Z. latifolia. Based on their biological functions, postharvest senescence of Z. latifolia may be closely associated with accelerated carbohydrate hydrolysis as well as strengthened PPP and weakened glycolysis and OXPHOS, leading to reduced energy supply and aggravated oxidative damage and consequently activating Ca2+/MAPKs, cytochrome c and jasmonate signaling pathways to cause primary metabolic disturbance and an increase in the accumulation of secondary metabolites such as lignin and consequently induce cell apoptosis or necrosis and finally accelerate senescence.

Key words: Zizania latifolia, mitochondria, isobaric tags for relative and absolute quantification, bioinformatics, senescence

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