食品科学 ›› 2019, Vol. 40 ›› Issue (9): 159-165.doi: 10.7506/spkx1002-6630-20180220-177

• 营养卫生 • 上一篇    下一篇

大果阿魏石油醚提取物成分分析及其对胃黏膜保护作用的评价

仲超逸1,2,田洪磊2,*,詹 萍2,*,王 鹏1,2,周文杰1,2   

  1. 1.石河子大学食品学院,新疆 石河子 832000;2.陕西师范大学食品工程与营养科学学院,陕西 西安 710119
  • 出版日期:2019-05-15 发布日期:2019-05-28
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0400705)

Component Analysis of Petroleum Ether Extract from Ferula lehmannii Boiss and Evaluation of Its Protective Effect on Gastric Mucosal Injury

ZHONG Chaoyi1,2, TIAN Honglei2,*, ZHAN Ping2,*, WANG Peng1,2, ZHOU Wenjie1,2   

  1. 1. College of Food, Shihezi University, Shihezi 832000, China; 2. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China
  • Online:2019-05-15 Published:2019-05-28

摘要: 目的:明晰新疆大果阿魏石油醚提取物中的物质组成及其对乙酸损伤型大鼠胃黏膜的保护作用,为胃黏膜保护及抗氧化类功能食品的开发提供借鉴。方法:采用乙酸所致大鼠胃黏膜损伤模型,将60 只SD大鼠分为正常组、模型组、阳性组(雷尼替丁0.03 g/kg),大果阿魏石油醚提取物分为高、中、低剂量组(1.0、0.2、0.04 g/kg mb),均连续灌胃两周,并采用气相色谱-质谱联用技术分析大果阿魏石油醚提取物中的化学成分。修复效果以胃黏膜损伤面积变化为评价指标,并计算损伤抑制率。同时,测定胃组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、前列腺素E2(prostaglandin E2,PGE2)及一氧化氮(NO)含量。结果:大果阿魏石油醚提取物中共分离鉴定出25 种物质,其中萜类化合物含量最多,相对含量为49.47%,其次是含硫化合物,相对含量为24.01%。各剂量大果阿魏石油醚提取物组均能明显改善大鼠胃黏膜损伤程度,与其他实验组相比,低、中、高剂量组损伤面积平均减小了7.68~13.10 mm2;与模型组相比,高剂量提取物组效果最佳,损伤抑制率达到31.84%,SOD活力增加了37.27%,MDA含量降低了32.51%,PGE2和NO含量分别增加了22.41%和28.13%。结论:大果阿魏石油醚提取物对大鼠乙酸性胃黏膜损伤具有明显的保护作用,作用机制可能与其提高胃黏膜抗氧化能力有关。

关键词: 大果阿魏提取物, 气相色谱-质谱联用, 成分分析, 胃黏膜损伤, 抗氧化活性

Abstract: Purpose: The present study aimed to characterize the chemical profile of petroleum ether extract from Ferula lehmannii Boiss and evaluate its gastroprotective activity, so as to provide an experimental basisfor the development of functional foods with antioxidant activity and gastric mucosal protection. Methods: The gastroprotective activity of Ferula lehmannii Boiss was investigated using an animal model of gastric ulcer induced by acetic acid. Totally 60 rats were divided into normal, model, ranitidine (positive control, at 0.03 g/kg), and high, medium and low-dose petroleum ether extract groups (1.0, 0.2 and 0.04 g/kg mb). The components of the extract were analyzed by gas-phase chromatography-mass spectrometry (GC-MS). The administration lasted for 2 weeks. At the end of the experimental period, all rats were sacrificed to collect stomach samples for evaluation of antioxidant indexes including superoxide dismutase (SOD) activity and malondialdehyde (MDA) level, and protective factors of gastric mucosa such as prostaglandin E2 (PGE2) and nitric oxide (NO). The change in gastric mucosal injury area was considered to evaluate the protective effect of the extract against gastric mucosal injury, and the percentage of injury inhibition was calculated. Results: Totally 25 compounds were identified from the extract, including terpenoids (49.47%) and sulphur-containing compounds (24.01%). The petroleum ether extract significantly attenuated gastric mucosal damage and reduced lesion area by 7.68 to 13.10 mm2 on average compared with other experimental groups. Compared with the model group, the extract at the high dose gave the maximum percentage of injury inhibition of 31.84%, increased SOD activity by 37.27%, decreased MDA level by 32.51%, and augmented the contents of PEG2 and NO by 22.41% and 28.13%. Conclusion: The petroleum ether extract from Ferula lehmannii Boiss can exert protective effect against chronic gastric mucosal injury, and the mechanism may be associated with the enhancement of antioxidant capacity.

Key words: extract from Ferula lehmannii Boiss, gas chromatography-mass spectrometry, component analysis, gastric mucosal injury, antioxidant activity

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