食品科学 ›› 2023, Vol. 44 ›› Issue (18): 93-101.doi: 10.7506/spkx1002-6630-20221223-227

• 生物工程 • 上一篇    

1-辛烯-3-醇对禾谷镰刀菌的抑制活性及作用机理

钱沈安，胡政，于伊楠，孟佳佳，张志岐，黄晴雯，赵志辉，聂冬霞，韩铮，范楷   

  1. （1.上海海洋大学食品学院，上海 201306；2.上海市农业科学院农产品质量标准与检测技术研究所，上海 201403；3.上海理工大学健康科学与工程学院，上海 200093）
  • 发布日期:2023-09-29
  • 基金资助:
    国家自然科学基金面上项目（32272442）

Inhibition Activity and Mechanism of 1-Octen-3-ol on the Growth and Deoxynivalenol Biosynthesis of Fusarium graminearum

QIAN Shen’an, HU Zheng, YU Yinan, MENG Jiajia, ZHANG Zhiqi, HUANG Qingwen, ZHAO Zhihui, NIE Dongxia, HAN Zheng, FAN Kai   

  1. (1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;2. Institute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 3. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
  • Published:2023-09-29

RichHTML

19

PDF (PC)

78

摘要： 以禾谷镰刀菌（Fusarium graminearum）PH-1为研究对象，研究1-辛烯-3-醇熏蒸对菌株生长和脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）生物合成的抑制作用，并通过考察其对镰刀菌细胞膜完整性、氧化应激水平以及DON合成关键基因表达等的影响，探讨可能涉及的抑制机理。结果表明，1-辛烯-3-醇熏蒸处理能显著抑制镰刀菌的菌落生长、孢子萌发和DON合成（P＜0.05），并呈现剂量依赖效应。100 μL/L（空气计）的1-辛烯-3-醇熏蒸7 d后，菌落直径、孢子萌发和DON产量的抑制率分别为60.70%、100.00%和97.50%。进一步实验发现1-辛烯-3-醇熏蒸处理有效破坏了禾谷镰刀菌细胞膜完整性和细胞膜通透性，造成麦角固醇含量下降，细胞内容物严重泄漏；此外，1-辛烯-3-醇可导致禾谷镰刀菌氧化应激平衡受到改变，TRI4、TRI5、TRI8、TRI10、TRI12和TRI101等DON合成关键基因显著下调。综上所述，1-辛烯-3-醇能有效抑制禾谷镰刀菌菌丝生长、孢子萌发和DON生物合成，主要通过影响细胞膜完整性、引起氧化应激和调控相关基因表达等产生抑制作用。

关键词: 1-辛烯-3-醇；禾谷镰刀菌；脱氧雪腐镰刀菌烯醇；抑制机理

Abstract: The inhibitory effect of fumigation with 1-octen-3-ol against the growth and deoxynivalenol (DON) biosynthesis of Fusarium graminearum PH-1 was determined, and the possible mechanism involved was investigated by evaluating cell membrane integrity, oxidative stress level and the expression of key genes related to DON biosynthesis. The results showed that 1-octen-3-ol significantly inhibited the mycelial growth, spore germination and mycotoxin production of F. graminearum in a concentration-dependent manner (P < 0.05). After 7 days of fumigation with 100 μL/L 1-octen-3-ol, the inhibition rates of mycelial growth, spore germination and deoxynivalenol production were 60.70%, 100.00% and 97.50%, respectively. Further studies showed that 1-octen-3-ol treatment effectively damaged the cell membrane integrity and increased the membrane permeability of F. graminearum, leading to a significant reduction of ergosterol levels and serious leakage of cell contents. Moreover, 1-octen-3-ol interfered with the oxidative stress balance of F. graminearum and downregulated the expression of DON biosynthesis-related genes such as TRI4, TRI5, TRI8, TRI10, TRI12, and TRI101. In conclusion, 1-octen-3-ol can effectively inhibit the growth and DON biosynthesis of F. graminearum by damaging cell membrane integrity, interfering with oxidative stress balance and regulating key gene expression.

Key words: 1-octen-3-ol; Fusarium graminearum; deoxynivalenol; inhibitory mechanism

中图分类号: 

京ICP备14027880号-3　京公网安备 11010202009729号
地址：北京市丰台区洋桥70号 中国食品杂志社《食品科学》编辑部　邮编：100068
电话：010-83155446/47/48/49/50　 传真：010-83155436　 Email：foodsci@126.com
本系统由北京玛格泰克科技发展有限公司设计开发　技术支持：support@magtech.com.cn