食品科学 ›› 2009, Vol. 30 ›› Issue (17): 253-257.doi: 10.7506/spkx1002-6630-200917058

• 生物工程 • 上一篇    下一篇

搅拌和溶氧对黄原胶发酵的影响

李增胜,张 庆,徐世艾*   

  1. 烟台大学 化工制造工程山东省高校重点实验室
  • 收稿日期:2008-09-11 修回日期:2008-12-26 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 徐世艾* E-mail:czs@ytu.edu.cn
  • 基金资助:

    国家自然科学基金项目(20476086;10272093);国家“863”计划项目(2006A030203)

Effects of Agitator Speed and Dissolved Oxygen on Xanthan Fermentation

LI Zeng-sheng,ZHANG Qing,XU Shi-ai*   

  1. Shandong Provincial Key Laboratory of Chemicals Manufacture Engineering, Yantai University, Yantai 264005, China
  • Received:2008-09-11 Revised:2008-12-26 Online:2009-09-01 Published:2014-04-14
  • Contact: XU Shi-ai* E-mail:czs@ytu.edu.cn

摘要:

利用实验室30L 发酵罐,在对pH 值控制的前提下,对黄单胞杆菌XG-101 的黄原胶间歇生产和生长动力学进行研究,发酵在一系列的搅拌速度(140~260r/min)下进行,测定产品的丙酮酸含量和黄原胶平均分子量。结果显示:随着搅拌转速的增加,发酵动力学各项参数值均增加,同时,在发酵过程中黄原胶的生成也与微生物的生长有一定的关系。另外,本实验通过新型微孔气体分布器改变溶氧,对黄原胶发酵的影响作了研究,结果发现用新型气体分布器所得黄原胶浓度较传统气体分布器下所得黄原胶浓度提高17%。

关键词: 黄原胶发酵, 平均分子质量, 丙酮酸功能基团, HPLC, 最大叶片式, 气体分布器

Abstract:

The kinetics of growth and xanthan production by Xanthomonas campestris XG-101 in batch culture were studied in a 30 L laboratory fermenter with pH control. Fermentations were carried out over a range of stirrer speed (140-260 r/min) and the content of pyruvic acid functional groups and the average molecular weight of the product obtained were determined. Increased agitation speed resulted in higher kinetics parameters of xanthan fermentation and product formation during the fermentation appeared to be partially associated with the strain growth. In addition, the effects of change of dissolved oxygen controlled by a new-type gas distributor on xanthan fermentation were studied. The results indicated that the xanthan concentration using the gas distributor was higher 17% than that using the traditional gas distributor.

Key words: xanthan fermentation, average molecular weight, pyruvic acid, HPLC, maxblend, gas distributor

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