食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 206-208.doi: 10.7506/spkx1002-6300-200918043

• 工艺技术 • 上一篇    下一篇

玉米须总黄酮的提取与抗氧化活性研究

方 敏,占才贵,宫智勇*   

  1. 武汉工业学院食品科学与工程学院
  • 收稿日期:2009-06-20 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 宫智勇 E-mail:zhiyong_gong@yahoo.com.cn

Extraction and Antioxidant Activities of Total Flavonoids from Corn Silk

FANG Min,ZHAN Cai-gui,GONG Zhi-yong*   

  1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
  • Received:2009-06-20 Online:2009-09-15 Published:2010-12-29
  • Contact: GONG Zhi-yong E-mail:zhiyong_gong@yahoo.com.cn

摘要:

以乙醇体积分数、浸提时间、固液比、提取温度为考察因素对玉米须总黄酮的提取条件进行正交设计试验,并用DPPH 和FRAP 两种方法测定了总黄酮的抗氧化活性。结果表明:玉米须总黄酮提取的最佳工艺条件为乙醇体积分数40%、提取时间2h、固液比1:25(m/V)、提取温度80℃,此时玉米须总黄酮的提取率为1.67%;玉米须总黄酮的抗氧化作用明显,对DPPH 自由基的清除率达到77.18%,总抗氧化能力FRAP 值为691.04μmol/L。

关键词: 玉米须, 总黄酮, 提取, 抗氧化

Abstract:

Total flavonoids in corn silk were extracted with ethanol and analyzed for their DPPH scavenging activity and total antioxidant capacity. The effects of ethanol concentration, extraction time, material/liquid ratio and extraction temperature were evaluated for optimizing the extraction procedure of total flavonoids. A maximum yield of total flavonoids of 1.67% was obtained by the 2 h extraction using 40% ethanol at 80 ℃. The total flavonoids from corn silk had marked antioxidant activities: the scavenging rate against DPPH free radicals was as high as 77.18% and the FRAP value reached 691.04 μmol/L.

Key words: corn silk, total flavonoids, extraction, antioxidant activities

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