食品科学 ›› 2009, Vol. 30 ›› Issue (11 ): 194-196.doi: 10.7506/spkx1002-6630-200911043

• 生物工程 • 上一篇    下一篇

木瓜蛋白酶对胰岛素蛋白残渣的水解

乔德阳,甘 聃   

  1. 徐州工业职业技术学院化学工程系
  • 收稿日期:2009-03-03 出版日期:2009-06-01 发布日期:2010-12-29
  • 通讯作者: 乔德阳, E-mail:qiaodeyang@sohu.com.cn

Papain-catalyzed Hydrolysis of Porcine Residues after Insulin Extraction

QIAO De-yang,GAN Dan   

  1. (Department of Chemical Engineering, Xuzhou College of Industrial Technology, Xuzhou 221140, China)
  • Received:2009-03-03 Online:2009-06-01 Published:2010-12-29
  • Contact: QIAO De-yang, E-mail:qiaodeyang@sohu.com.cn

摘要:

对胰岛素提取残渣样品进行初步分析,并研究木瓜蛋白酶对胰岛素蛋白残渣水解条件。结果表明,该残渣中各成分含量为粗脂肪质量含量为7.15%、水分32.20%、蛋白质50.49%、灰分5.58%。残渣样品经过高温处理20min,固液比为0.6(W/V),木瓜蛋白酶添加量为0.6%(W/V),酶解时间8h,酶解温度为55℃时,残渣的水解度可以达到41.25%。

关键词: 木瓜蛋白酶, 胰岛素蛋白残渣, 水解度

Abstract:

Porcine pancreatic residue, remaining after the extraction of insulin, was subjected to primary chemical component analysis and hydrolysis by papain. The results showed that the residues contained fat of 7.15%, water of 32.20%, protein of 50.49%, and ash of 5.58%. The optimal conditions of hydrolysis were as follows: after high temperature treatment for 20 min, with the ratio of solid to liquid of 0.6 and papain amount of 0.6%, hydrolysis at 55 ℃ for 8 h. Under this condition, the degree of hydrolysis was up to 41.25%.

Key words: papain, porcine reside after insulin extraction, degree of hydrolysis

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