食品科学 ›› 2006, Vol. 27 ›› Issue (8): 79-82.

• 基础研究 • 上一篇    下一篇

费氏丙酸杆菌费氏亚种在不同基质中转化生成共轭亚油酸的研究

 王丽敏, 吕加平   

  1. 中国农业科学院农产品加工研究所
  • 出版日期:2006-08-15 发布日期:2011-10-14

Production of Conjugated Linoleic Acids by Propionibacterium freudenreichii ssp.freudenreichii in Different Media

 WANG  Li-Min, 吕Jia-Ping   

  1. Institute of Agro-Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100094,China
  • Online:2006-08-15 Published:2011-10-14

摘要: 研究了在MRS、乳酸钠和脱脂乳三种培养基质下温度、时间、底物浓等因素对P.freudenreichiissp.freudenreichii生成CLA的影响,结果表明P.freudenreichiissp.freudenreichii在MRS、乳酸钠和脱脂乳中均具有生成CLA的能力,三种培养基质中一定浓度的葵花籽油对P.freudenreichiissp.freudenreichii的生长都有明显的抑制作用,在MRS培养基中葵花籽油浓度为12mg/ml,菌液量10%(V/V),30℃培养24hCLA生成量最大为24.813μg/ml。

关键词: 共轭亚油酸, 费氏丙酸杆菌费氏亚种, MRS乳酸钠培养基, 脱脂乳培养基

Abstract: The effects of temperature,time and sunflower oil level on the formation of conjugated linoleic acids(CLA)by P.freudenreichii ssp.freudenreichii in MRS/SLM and skim-milk were investigated.The formation of CLA in three media was observed and the growth of P.freudenreichii ssp.freudenreichii was inhibited by sunflower oil in the media.Inoculation of P.freudenreichii ssp.freudenreichii into MRS medium under anaerobic conditions for 24h incubation is most effective in promoting CLA formation.

Key words:  , conjugated linoleic acids; P.freudenreichii ssp.freudenreichii; MRS; SLM; skim-milk;