食品科学 ›› 2003, Vol. 24 ›› Issue (1): 107-110.

• 分析检测 • 上一篇    下一篇

向日葵籽中酚酸含量的高效液相色谱测定方法研究

 陈少洲, 吕飞杰, 台建祥   

  1. 中国农业科学院农产品加工中心
  • 出版日期:2003-01-15 发布日期:2011-12-13

Study on Determination of Phenolic Acid in Sunflower Seeds by HPLC

 CHEN  Shao-Zhou, LU Fei-Jie , TAI  Jian-Xiang   

  • Online:2003-01-15 Published:2011-12-13

摘要: 采用ALLTIMACl8色谱柱(250mm×4.6mmID,5μm),乙腈∶2%冰醋酸溶液(15∶85)为流动相,流速为1.0ml/min,SPD-6AV紫外检测器,柱温为室温,检测波长327nm。建立了测定向日葵籽中酚酸含量的高效液相色谱法,同时测定了部分向日葵品种中酚酸的含量。

关键词: 高效液相色谱, 向日葵籽, 酚酸

Abstract: A method for determination phenolic acid(chlorogenic acid and caffeic acid)in sunflower seeds by HPLC was described.Chromatographi c separation was performed ALLTIMA C 18 column (250mm ×4.6mm ID,5μm)byusingan acetonitrile -2%glacicalaceticacid(15∶85,v /v)mixtureas the mobilephase andusing SPD -6AVultraviolet detection at 327nmwith a flowrate of l.0ml /min.The chlorogenic acid and caffeic acid were eluted in 18min with satisfacto ry separation and the content of phen olic acid in different genotypes of sun-flower was also determinded.

Key words: HPLC, sunflower seed, phenolic acid