食品科学 ›› 2012, Vol. 33 ›› Issue (24): 147-151.doi: 10.7506/spkx1002-6630-201224030

• 工艺技术 • 上一篇    下一篇

榆耳菌丝体多糖提取及脱色条件的研究

张公亮,王应男,刘洋,张浩,侯红漫*   

  1. 大连工业大学食品学院,辽宁 大连 116034
  • 收稿日期:2011-09-24 修回日期:2012-11-21 出版日期:2012-12-25 发布日期:2012-12-12
  • 通讯作者: 王应男 E-mail:510516yngl@163.com

Extraction and Decolorization of Mycelial Polysaccharides from Gloeostrereum incarnatum

ZHANG Gong-liang,WANG Ying-nan,LIU Yang,ZHANG Hao,HOU Hong-man*   

  1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
  • Received:2011-09-24 Revised:2012-11-21 Online:2012-12-25 Published:2012-12-12

摘要:

对榆耳菌丝体多糖提取条件和脱色条件进行研究,以提高产品的得率和纯度。通过单因素及正交试验,得到榆耳菌丝体胞内多糖的最佳提取条件:水料比40:1、提取时间8h、提取温度95℃、提取次数3次。在此条件下,榆耳菌丝体胞子内多糖得率为8.60%。采用AB-8大孔树脂对多糖脱色,最佳条件为添加量0.2g/mL、pH4.0、温度60℃。

关键词: 榆耳, 菌丝体, 多糖, 提取, 脱色

Abstract:

Orthogonal array design was used to optimize conditions for the extraction and de-colorization of mycelial polysaccharides from Gloeostereum incarnatum to obtain enhanced yield and purity. The optimum extraction conditions were found to be three extraction cycles at 95 ℃ for 8 h with a water-to-solid ratio of 40:1. Under these conditions, the polysaccharide yield was 8.60%. AB-8 resin was used to decolorize the crude polysaccharide extract, and the optimum decolorization conditions were found to be a solid-to-liquid ratio of 0.2 g/mL, pH 4.0 and 60 ℃.

Key words: Gloeostereum insarnatum, mycelium, polysaccharides, extraction, de-colorization

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