二氢杨梅素对小鼠运动性疲劳的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 295-297.

二氢杨梅素对小鼠运动性疲劳的影响

高辉，李春艳*

吉首大学医学院药理学教研室

收稿日期:2012-06-08 修回日期:2012-10-23 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 李春艳 E-mail:lchy1965@163.com
基金资助:
湖南省教育厅科研项目;湖南省教育厅教改资助项目

Effect of Dihydromyricetin on Exercise-induced Fatigue in Mice

Received:2012-06-08 Revised:2012-10-23 Online:2012-11-15 Published:2012-11-09

摘要/Abstract

摘要： 目的：研究二氢杨梅素(DMY)对运动耐力的影响。方法：设计5组实验，每个实验均取40只小鼠随机分成DMY高(300mg/(kg·d))、中(200mg/(kg·d))、低(100mg/(kg·d))剂量组及对照组(蒸馏水)，每天给药一次。处理一周后分别进行负重游泳实验、耐缺氧实验，并检测相应指标，检测运动后乳酸变化、尿素氮变化及肝糖原、肝组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力的变化。结果：DMY处理各组小鼠负重游泳时间及高、中剂量组小鼠缺氧生存时间较对照组明显延长(P＜0.05)，肝糖原含量高于对照组(P＜0.01)，血清乳酸、尿素氮含量则低于对照组(P＜0.05或P＜0.01)；肝组织MDA含量低于对照组(P＜0.01)，但各组间SOD活力无显著性差异。结论：DMY具有抗疲劳作用，这一作用可能与其抑制组织的过氧化水平有关。

关键词: 二氢杨梅素, 运动性疲劳, 负重游泳, 耐缺氧, 抗氧化

Abstract: Objective: To explore the anti-fatigue effect of dihydromyricetin (DMY) in mice. Methods: A total of 40 male mice were randomly divided equally into high-dose DMY group (300 mg/kg), middle-dose DMY group (200 mg/kg), low-dose DMY group (100 mg/kg) and control group (distilled water). The mice were intragastrically administered the same volume of DMY solution or distilled water once daily for one successive week. Anti-hypoxia and weight-bearing swimming tests were carried out to analyze changes in serum lactic acid and urea nitrogen contents and liver glycogen content, MDA content and SOD activity before and after exercise. Results: Compared with the control group, weight-bearing swimming time was significantly prolonged by DMY administration at three doses, while a significant prolongation in anoxia survival time was observed for only high-dose and middle-dose groups (P＜0.05); in addition, both groups revealed a significantly higher level of liver glycogen (P＜0.01) but significant lower levels of serum lactic acid and urea nitrogen (P＜0.05 or P＜0.01) and liver MDA (P＜0.01), whereas there was no significant difference in SOD activity between the two DMY groups. Conclusion: DMY has an obvious anti-fatigue effect, which may be related to the inhibition of lipid peroxidation in liver tissue.

Key words: dihydromyricetin, exercise-induced fatigue, weight-bearing swimming, anti-anoxia, antioxidation

中图分类号:

TS275.4

高?辉，李春艳* . 二氢杨梅素对小鼠运动性疲劳的影响[J]. 食品科学, 2012, 33(21): 295-297.

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