UPLC法评价多种人参提取物中人参皂苷的含量

doi:10.7506/spkx1002-6630-201324021

食品科学

UPLC法评价多种人参提取物中人参皂苷的含量

张玉婷，冯克然，曹进，王静文，张庆生，丁丽霞，王钢力

1.中国食品药品检定研究院食品化妆品检定所，北京 100050；2.中国药学会，北京 100022

出版日期:2013-12-25 发布日期:2014-01-03

Determination of Eight Ginsenosides in Ginseng Extracts by UPLC

ZHANG Yu-ting，FENG Ke-ran，CAO Jin，WANG Jing-wen，ZHANG Qing-sheng，DING Li-xia，WANG Gang-li

1. Institute for Food and Cosmetic Control, National Institutes for Food and Drug Control, Beijing 100050, China；
2. Chinese Pharmaceutical Association, Beijing 100022, China

Online:2013-12-25 Published:2014-01-03

摘要/Abstract

摘要：

利用超高效液相色谱法测定用于保健食品原料的人参提取物中8种人参皂苷的含量，并对测定的13个厂家的44批人参提取物中人参皂苷的含量进行评价。方法采用Waters ACQUITY UPLC® BEH C18(50mm×2.1mm，1.7μm)色谱柱，柱温30℃，乙腈-水为流动相梯度洗脱，流速0.5mL/min，203nm波长处检测。结果表明：8种人参皂苷在15min内基线分离，线性良好(r=0.9990～0.9997)。最低检出量为0.47～1.96ng。回收率在95.0%～110.0%。该方法快速简便、稳定可靠，可作为人参提取物中8种人参皂苷含量的快速测定方法。

关键词: 超高效液相色谱, 人参提取物, 人参皂苷

Abstract:

A method for rapid determination of 8 ginsenosides in ginseng extracts of different sources using ultra
performance liquid chromatography (UPLC) was established. The content of ginsenosides in 44 batches of ginseng extracts
from 13 sources was analyzed and evaluated. The separation was performed on a Waters ACQUITY UPLC BEH® C18
column (50 mm×2.1 mm, 1.7 μm) at 30 ℃ at a flow rate of 0.5 mL/min through gradient elution with acetonitrile-water
mixture and detection at a wavelength of 203 nm. Eight ginsenosides were well separated within 15 min with correlation
coefficients of 0.9990–0.9997. The detection limits for ginsenosides were 0.47-1.96 ng with recoveries ranging from 95.0%
to 110.0%. The method was simple, rapid, and reliable, and thus, was suitable for the rapid determination of 8 ginsenosides
in ginseng extracts from different sources.

Key words: ultra performance liquid chromatography (UPLC), ginseng extracts, ginsenosides

张玉婷，冯克然，曹进，王静文，张庆生，丁丽霞，王钢力. UPLC法评价多种人参提取物中人参皂苷的含量[J]. 食品科学, doi: 10.7506/spkx1002-6630-201324021.

ZHANG Yu-ting，FENG Ke-ran，CAO Jin，WANG Jing-wen，ZHANG Qing-sheng，DING Li-xia，WANG Gang-li. Determination of Eight Ginsenosides in Ginseng Extracts by UPLC[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201324021.

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UPLC法评价多种人参提取物中人参皂苷的含量

Determination of Eight Ginsenosides in Ginseng Extracts by UPLC

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