肉鸭表皮组织中脱氢枞酸残留的SPE-HPLC检测方法

doi:10.7506/spkx1002-6630-201416019

食品科学

肉鸭表皮组织中脱氢枞酸残留的SPE-HPLC检测方法

张苏珍1,2，耿志明2,*，王道营2，诸永志2，刘芳2，张牧焓2，卞欢2，徐为民2

1.南京农业大学肉品加工与质量控制教育部重点实验室，江苏南京 210095；
2.江苏省农业科学院农产品加工研究所，江苏南京 210014

出版日期:2014-08-25 发布日期:2014-08-25
通讯作者: 耿志明
基金资助:
国家自然科学基金青年科学基金项目（31101312）；江苏省农业科技自主创新资金项目（CX(13)3081）

Development of SPE-HPLC Method for Determination of Dehydrogenated Abietic Acid Residue in Duck Skin Tissue

ZHANG Su-zhen1,2, GENG Zhi-ming2,*, WANG Dao-ying2, ZHU Yong-zhi2, LIU Fang2, ZHANG Mu-han2, BIAN Huan2, XU Wei-min2

1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University,
Nanjing 210095, China; 2. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Online:2014-08-25 Published:2014-08-25
Contact: GENG Zhi-ming

摘要/Abstract

摘要：

建立经松香脱毛处理的肉鸭表皮组织中残留脱氢枞酸的固相萃取-高效液相色谱检测方法。肉鸭表皮组织中的脱氢枞酸用乙腈提取，经C18固相萃取小柱净化，以0.003 mol/L磷酸溶液-甲醇（13∶87，v/v）为流动相，采用反相C18（250 mm×4.6 mm，5 μm）色谱柱分离，以紫外检测器（检测波长212 nm）进行检测。结果表明：该方法检测脱氢枞酸线性范围为0.1～10 mg/L，检测限为0.10 μg/g，定量限为0.33 μg/g。在低中高3 个添加量范围内回收率为80.8%～91.8%。实际样品分析显示，肉鸭表皮中脱氢枞酸含量最高达10.06 μg/g。所建立的分析方法简便快捷、具有较好的灵敏度和可靠性，可用于肉鸭表皮组织中脱氢枞酸残留量的分析。

关键词: 肉鸭, 松香, 脱氢枞酸, 高效液相色谱法, 固相萃取

Abstract:

A high-performance liquid chromatographic (HPLC) method was established to determine dehydrogenated abietic
acid in duck skin. Dehydrogenated abietic acid in duck skin sample was extracted with acetonitrile, followed by purification
with a C18 solid-phase extraction (SPE) cartridge, and detected by HPLC-ultraviolet detector (UV). The chromatographic
conditions were as follows: column, reversed-phase C18 (250 mm × 4.6 mm, 5 μm); mobile phase, a mixture of 0.003 mol/L
phosphoric acid-methanol (13:87, V/V); flow rate, 1.0 mL/min; and detection wavelength, 212 nm. Results indicated that
the linearity ranged from 0.1 to 10.0 mg/L, and the detection limit and the quantification limit were 0.10 μg/g and 0.33 μg/g,
respectively. The recoveries of dehydrogenated abietic acid spiked at 0.5–20 μg/g were in the range of 80.8%–91.8%. The
method was successfully applied for the determination of dehydrogenated abietic acid in rosin-defeathered duck skin, and
the result indicated that content of dehydrogenated abietic acid in the duck sample could reach 10.06 μg/g. The present
method proved to be of high maneuverability and excellent sensitivity and accuracy, and could be employed to analyze
dehydrogenated abietic acid residue in duck skin tissue.

Key words: duck, rosin, dehydrogenated abietic acid, high-performance liquid chromatographic (HPLC), solid polymer electrolyte (SPE)

中图分类号:

TS201.5

张苏珍1,2，耿志明2,*，王道营2，诸永志2，刘芳2，张牧焓2，卞欢2，徐为民2. 肉鸭表皮组织中脱氢枞酸残留的SPE-HPLC检测方法[J]. 食品科学, doi: 10.7506/spkx1002-6630-201416019.

ZHANG Su-zhen1,2, GENG Zhi-ming2,*, WANG Dao-ying2, ZHU Yong-zhi2, LIU Fang2, ZHANG Mu-han2, BIAN Huan2, XU Wei-min2. Development of SPE-HPLC Method for Determination of Dehydrogenated Abietic Acid Residue in Duck Skin Tissue[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201416019.

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肉鸭表皮组织中脱氢枞酸残留的SPE-HPLC检测方法

Development of SPE-HPLC Method for Determination of Dehydrogenated Abietic Acid Residue in Duck Skin Tissue

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