食品科学

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响应面优化超临界CO2萃取八月瓜幼果多酚工艺

顾仁勇,杨万根,余 佶   

  1. 吉首大学化学化工学院,湖南 吉首 416000
  • 出版日期:2015-05-25 发布日期:2015-05-08

Optimization of Supercritical CO2 Extraction Conditions of Polyphenols from Young Fruits of Holboellio latifolia by Response Surface Methodology

GU Renyong, YANG Wangen, YU Ji   

  1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China
  • Online:2015-05-25 Published:2015-05-08

摘要:

为了综合利用八月瓜幼果资源,采用超临界CO2萃取技术提取八月瓜多酚。探讨了不同体积分数乙醇溶液作为夹带剂对多酚的萃取效果,采用响应面法,以多酚萃取得率为响应值,考察原料与夹带剂的料液比、萃取压力和萃取温度对多酚萃取的影响,并优化萃取条件。结果表明:以体积分数95%乙醇溶液为夹带剂,可使八月瓜幼果多酚萃取得率提高约2.45 倍,产品纯度提高约15.49%;优化所得最佳条件为料液比1∶2.6(g/mL)、萃取压力37 MPa、萃取温度51 ℃,此条件下八月瓜幼果多酚萃取得率可达6.41%,产品纯度为87.69%。

关键词: 八月瓜幼果, 多酚, 超临界CO2萃取, 夹带剂, 响应面优化法

Abstract:

Polyphenols were extracted from the young fruits of Holboellio latifolia by supercritical CO2 extraction. The
effect of ethanol concentration on extraction efficiency was examined. The extraction conditions solid/liquid ratio (namely
material/entrainer ratio), extraction pressure and temperature were investigated and the optimum extraction conditions were
obtained by response surface methodology. The results showed that the extraction yield and purity of polyphenols were
enhanced by 2.45 times and 15.49%, respectively, when using 95% ethanol as the entrainer, in comparison to those obtained
without the entrainer. The optimum extraction conditions for solid/liquid ratio, extraction pressure and temperature were 1:2.6
(g/mL), 37 MPa and 51 ℃, respectively. Under these conditions, the extraction yield and purity of polyphenols reached 6.41%
and 87.69%, respectively.

Key words: young fruits of Holboellio latifolia, polyphenols, supercritical CO2 extraction, entrainer, optimization by response surface methodology

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