食品科学 ›› 2011, Vol. 32 ›› Issue (9): 285-289.doi: 10.7506/spkx1002-6630-201109063

• 营养卫生 • 上一篇    下一篇

饲喂羊肉和鸭肉对大鼠水盐代谢影响的比较研究

闫振国,冯宪超,徐幸莲*,周光宏   

  1. 南京农业大学 教育部肉品加工与质量控制重点实验室
  • 出版日期:2011-05-15 发布日期:2011-04-11

Comparative Effects of Diets Supplemented with Sheep and Duck Meat on Water and Salt Metabolism in Rats

YAN Zhen-guo,FENG Xian-chao,XU Xing-lian*,ZHOU Guang-hong   

  1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2011-05-15 Published:2011-04-11

摘要: 目的:探索饲喂羊肉和鸭肉对大鼠水盐代谢的影响。方法:70只3周龄雄性SD大鼠,预饲4d,断颈宰杀10只,采集血清备用,其余60只随机均分成羊肉饲料组、鸭肉饲料组、豆粕饲料(对照组)组。饲养期间测定各组大鼠的饮水量、采食量、粪便含水量、粪便硬度;分别在第14天和第28天宰杀各组大鼠的一半,断颈采血,采用放射免疫分析法(RIA)检测血清抗利尿激素(AVP)、醛固酮(ALD)水平,全自动生化分析仪测定血清钠离子(Na+)、钾离子(K+)、氯离子(Cl-)水平。结果:1)羊肉组大鼠的饮水量/单位体质量、采食量/单位体质量、粪便硬度都高于鸭肉组,粪便含水量低于鸭肉组。与鸭肉组相比,第7天单位体质量饮水量差异显著;2)相对于豆粕(对照组)组,羊肉组大鼠血清抗利尿激素(AVP)、醛固酮(ALD)含量升高,在第14天为(103.4±13.10)pg/mL、(2.02±0.37)ng/mL,第28天分别为(87.67±3.78)pg/mL、 (2.39±0.34)ng/mL,而鸭肉组降低,在第14天分别为(90.98±5.33)pg/mL、(1.67±0.32)ng/mL,第28天分别为(72.36±11.24)pg/mL、(1.89±0.35)ng/mL,且两组相比差异显著(第14天,P<0.05;第28天,P<0.01);3)羊肉组Na+含量在第14天低于鸭肉组,而在第28天高于鸭肉组,且差异有统计学意义(P<0.05);第14天时,羊肉组K+含量显著低于鸭肉组(P<0.05);第14天,豆粕对照组Cl-含量显著高于羊肉组和鸭肉组,钠钾比(Na+/K+)显著高于羊肉组,其他差异不显著,羊肉组和鸭肉组相比,Cl-、Na+/K+差异均无统计学意义(P>0.05)。结论:饲喂羊肉使大鼠机体AVP和ALD的分泌增强,向体外利水的生理作用受限,饲喂鸭肉在一定程度上降低机体AVP和ALD水平,使机体向外利水的生理作用增强。

关键词: 饲喂, 羊肉, 鸭肉, 豆粕, 水盐代谢

Abstract: Objective: To observe the effect of diet supplemented with sheep or duck meat on water and salt metabolism in rats. Methods: Totally 70 three-weeks male SD rats were used as experimental subjects. After feeding for 4 days, 10 rats were scarified to collect blood. The 60 others were randomly divided into three groups to feed with diet supplemented with sheep meat, duck meat and soybean. The fecal water content, stool hardness, water intake amount and food intake amount of rats from each group were determined during the feeding process. Ten rats from each group were killed to collect blood at the 14th and 28th day during the feeding period, respectively. Serum vasopressin (AVP) and aldosterone (ALD) were measured by radioimmunoassay (RIA), and Na+, K+ and Cl- were determined by automatic biochemical analyzer. Result: The stool hardness, water intake amount and food intake amount in rats fed sheep meat diet were higher than those in rats fed duck meat diet, but fecal water content in rats fed sheep meat diet was lower than that in rats fed duck meat diet. The two groups signicantly different in their water intake amounts at the 7th day. Compared with the soybean diet group, the serum levels of AVP and ALD exhibited an increase in sheep meat diet group, which reached up to (103.4 ± 13.10) pg/mL and (2.02 ± 0.37) ng/mL at the 14th day, and (87.67 ± 3.78) pg/mL and (2.39 ± 0.34) ng/mL at the 28th day; in contrast, exhibited a decrease in duck meat diet group, which were (90.98 ± 5.33) pg/mL and (1.67 ± 0.32) ng/mL at the 14th day, and (72.36 ± 11.24) pg/mL and (1.89 ± 0.35) ng/mL at the 28th day, respectively (at the 14th day, P<0.05; at the 28th day, P<0.01). In addition, a significant lower serum Na+ content in the sheep meat diet group was observed when compared with the duck meat group (P<0.05) after 14 days of feeding; in contrast, a higher level of serum Na+ in the sheep meat diet group was observed at the end of 28th day. Serum K+ was decreased significantly in sheep meat diet group when compared with the duck meat diet group (P < 0.05) in the feeding period of 14 days. Similarly, during the feeding period of 14 days, the serum Cl- in the soybean diet group were significantly higher than that of the sheep meat diet group and duck meat diet group; the ratio of Na+/ K+ in soybean diet group was significantly higher than the sheep meat group. Conclusion: These investigations suggested that sheep meat diet could increase AVP and ALD level, while duck meat diet could decrease AVP and ALD level to some extent. The physiological function of water release was limited during feeding period of sheep meat diet and increased during feeding period of duck meat diet.

Key words: feed, heep meat, duck meat, soybean, water and salt metabolism

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