食品科学 ›› 2011, Vol. 32 ›› Issue (15): 66-69.doi: 10.7506/spkx1002-6630-201115016

• 基础研究 • 上一篇    下一篇

锌对枸杞提取物抗氧化活性的影响

靳利娥,王晓娟,闫果兰*   

  1. 太原理工大学化学化工学院
  • 出版日期:2011-08-15 发布日期:2011-07-26
  • 基金资助:
    国家自然科学基金项目(30771576);山西省自然科学基金项目(2009011047)

Effect of Zinc on Antioxidant Activity of Lycium Extract

JIN Li-e,WANG Xiao-juan,YAN Guo-lan*   

  1. (College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China)
  • Online:2011-08-15 Published:2011-07-26

摘要: 针对天然提取物单独使用时其抗氧化活性不及人工合成抗氧化物强的问题,以氯化锌、葡萄糖酸锌、乙酸锌为原料与65%乙醇为溶剂超声波法提取的枸杞提取物进行络合反应,制备成复合物,采用油脂氧化法、β-胡萝卜素漂白法、清除DPPH自由基以及清除超氧阴离子自由基(O2- ·)4种方法评价复合物和枸杞提取物的抗氧化活性,通过比较其抗氧化性,考察金属元素锌存在下对枸杞提取物抗氧化性能的影响。由于抗氧化机理的不同,锌离子的存在对枸杞提取物的抗氧化活性均有一定影响,其中乙酸锌与枸杞提取物络合产物(LZn2-AR)清除DPPH自由基、O2- ·的能力最强,当络合物质量浓度为800mg/L时对DPPH自由基清除率可达85%,对O2- ·清除率可达20%;葡萄糖酸锌与枸杞提取物络合物(LZn3-AR)的抗油脂氧化能力最强;氯化锌与枸杞提取物络合物具(LZn1-AR)有抗β-胡萝卜素氧化的能力;利用可见-紫外光谱分析可知,氯化锌、葡萄糖酸锌、乙酸锌与枸杞提取物制备成的络合物均出现新的吸收峰,表明均形成新物质。

关键词: 枸杞, 提取物, 锌元素, 抗氧化性

Abstract: In order to solve the problem that the antioxidant activities of natural extracts are weaker than those of synthetic antioxidants, the effect of zinc ions on the antioxidant activity of lycium extract was explored. The antioxidant activity of ethanol extract from lycium in the presence of zinc chloride, zinc gluconate or zinc acetate was evaluated by lipid oxidation, β-carotene bleaching, DPPH radical scavenging capacity and superoxide anion radical scavenging capacity. Due to different antioxidant mechanisms, the antioxidant activity of ethanol extract from lycium could be affected by zinc ions. The complex between zinc acetate and lycium extract revealed the strongest scavenging capacity against DPPH and superoxide anion free radicals, with a scavenging rate of 85% and 20%, respectively when the complex concentration was 800 mg/L. The complex between zinc gluconate and lycium extract showed the strongest inhibitory effect against lipid oxidation. In contrast, the complex between zinc chloride and lycium extract was the strongest in inhibiting β-carotene oxidation. Different new absorption peaks in UV-visible spectra of these complexes were observed, suggesting that new substances were generated.

Key words: lycium extract, zinc ion, antioxidant activity

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