食品科学 ›› 2011, Vol. 32 ›› Issue (24): 83-87.doi: 10.7506/spkx1002-6630-201124018

• 工艺技术 • 上一篇    下一篇

脱脂麦胚水提液中谷胱甘肽的初步分离

宋国辉,黄纪念,孙强,张丽霞,芦鑫   

  1. 河南省农科院农副产品加工研究所,河南省农产品生物活性物质工程技术研发中心
  • 出版日期:2011-12-25 发布日期:2011-12-29
  • 基金资助:
    河南省农业科学院科研发展专项资金项目

Isolation of Glutathione from Aqueous Extract of Defatted Wheat Germ

SONG Guo-hui,HUANG Ji-nian,SUN Qiang,ZHANG Li-xia,LU Xin   

  1. (Institute of Agricultural Products Processing, Henan Academy of Agriculture Sciences, Henan Engineering Research Centre of Bioactive Substances in Agricultural Products, Zhengzhou 450002, China)
  • Online:2011-12-25 Published:2011-12-29

摘要: 对脱脂麦胚水提液中的谷胱甘肽的分离进行研究。通过对谷胱甘肽的稳定性进行研究以及比较不同纯化工艺组合对谷胱甘肽质量分数的影响,建立采用超滤结合离子交换树脂层析分离脱脂麦胚水提液中谷胱甘肽的工艺技术,结果所得产品中谷胱甘肽的质量分数由未纯化前的0.79%提高到8.8%,为进一步纯化谷胱甘肽提供了良好的方法。

关键词: 脱脂麦胚, 谷胱甘肽, 超滤, 离子交换

Abstract: The isolation of glutathione (GSH) from aqueous extract of defatted wheat germ (DWG) was explored. The effects of temperature and pH on the stability of GSH in aqueous extract of DWG were studied during. An isolation method for isolating GSH from aqueous extract of DWG was established using ultra-filtration followed by TOSO SP-650M ion-exchange resin chromatography. The GSH content in the isolated product was 8.8% compared with 0.79% in aqueous extract of DWG.

Key words: defatted wheat germ, glutathione, ultra-filtration, ion-exchange

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