食品科学 ›› 2012, Vol. 33 ›› Issue (13): 254-258.doi: 10.7506/spkx1002-6630-201213054

• 生物工程 • 上一篇    下一篇

不同浓度沙门氏菌细胞生长分布规律研究

周康,GEORGESusie,BARANYIJozsef,李平兰   

  1. 1.中国农业大学食品科学与营养工程学院 2. Institute of Food Research, Norwich Research Park, Norwich
  • 收稿日期:2020-04-01 修回日期:2020-04-01 出版日期:2012-07-15 发布日期:2012-07-27

Modeling the Growth Distribution of Salmonella at Different Inoculum Levels

ZHOUKang,GEORGESusie,BARANYIJozsef,LIPing-lan   

  1. (1. College of Food Science and Nutritional Engineering, China Agricultral University, Beijing 100083, China; 2. Institute of Food Research, Norwich Research Park, Norwich NR47UA, UK)
  • Received:2020-04-01 Revised:2020-04-01 Online:2012-07-15 Published:2012-07-27

摘要: 选用沙门氏菌SL1344在含20mg/kg甜菜碱和NaCl质量分数分别为0.0%、3.5% 和5.0%的最低营养培养基(BMM)环境中分别对大量细胞、少量细胞进行研究,并对3.5% NaCl条件下单个细胞的生长进行研究。重点介绍进行少量细胞研究的一般理论和方法,以及单个细胞研究中单细胞生长流动槽成像系统的应用。结果发现,大量细胞生长分布具有决定性,单细胞生长分布具有随机性的特点,并通过不同的生长模型初步阐述其关系。

关键词: 沙门氏菌, 生长模型, 单细胞, 单细胞生长流动槽, 随机分布, 决定分布

Abstract: The basic minimal media (BMM) containing 0%, 3.5% or 5% NaCl and 20 mg/kg betaine Salmonella SL1344 were used to culture Salmonella enterica SL 1344 at an inoculum level of 103 CFU/mL in a constant temperature oven or at lower inoculum gradients in a Bioscreen for growth curve investigations. In addition, the growth of single cells under the condition of 3.5% NaCl was explored. The results showed that the growth distribution of cell colonies was determinative. By contrast, the growth distribution of single cells was stochastic. The relationship between the growth of single cells and cell colonies could be modeled.

Key words: Salmonella enterica, growth model, single cell, flow chamber, stochastic distribution, determinative distribution

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