食品科学 ›› 2020, Vol. 41 ›› Issue (16): 110-116.doi: 10.7506/spkx1002-6630-20190703-033

• 生物工程 • 上一篇    

黑曲霉固态发酵对甘蔗叶酚类物质释放及抗氧化活性的影响

阎欲晓,粟桂娇,何勇强   

  1. (广西大学生命科学与技术学院,广西 南宁 530004)
  • 发布日期:2020-08-19
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660505)

Effects of Solid-state Fermentation with Aspergillus niger on Phenolics Release and Antioxidant Activity of Sugarcane Leaves

YAN Yuxiao, SU Guijiao, HE Yongqiang   

  1. (College of Life Science and Technology, Guangxi University, Nanning 530004, China)
  • Published:2020-08-19

摘要: 采用黑曲霉对甘蔗叶进行固态发酵,研究接种量、培养基含水量、发酵时间对甘蔗叶酚类物质释放的影响;对代谢过程中产生的纤维素酶、β-葡萄糖苷酶活性进行测定,并考察发酵前后甘蔗叶提取物抗氧化活力的变化。结果表明,发酵后甘蔗叶中多酚、黄酮含量显著提高。在接种量28 mL/100 g、培养基含水量50%条件下发酵5 d后,测得甘蔗叶中黄酮含量为13.82 mg/g(干基),多酚含量为10.40 mg/g(干基),比未发酵甘蔗叶中黄酮和多酚含量分别提高了135%和92%。纤维素酶活力在发酵第4天达到最大,为632.17 U/g;β-葡萄糖苷酶活力在发酵第5天时最高,为75.02 U/g。甘蔗叶发酵过程中酚类物质释放量与纤维素酶和β-葡萄糖苷酶活力呈正相关。通过计算IC50,确定发酵后甘蔗叶黄酮提取液对羟自由基和1,1-二苯基-2-三硝基苯肼自由基清除能力均比发酵前有较大提高。

关键词: 甘蔗叶;固态发酵;酚类物质;纤维素酶;β-葡萄糖苷酶;抗氧化活性

Abstract: Solid-state fermentation of sugarcane leaves was performed with Aspergillus niger for the purpose of extracting secondary metabolites. The effects of inoculum size, the water content of fermentation substrate and fermentation time on the release of phenolics from sugarcane leaves were investigated. The activities of cellulase and β-glucosidase were determined during the fermentation process. The antioxidant activity of the flavonoids extracted from native and fermented sugarcane leaves was investigated. The results showed that the contents of polyphenols and flavonoids in sugarcane leaves significantly increased after fermentation, reaching 10.40 and 13.82 mg/g on a dry basis under the following conditions: inoculum size 28 mL/100 g, water content of culture medium 50%, and fermentation time 5 days, which increased by 135% and 92% as compared with those in the unfermented samples, respectively. The activity of cellulase reached its maximum at 632.17 U/g on the fourth day of fermentation and the activity of β-glucosidase peaked at 75.02 U/g on the fifth day. Positive correlations were found between the contents of phenolic compounds and the activities of cellulase and β-glucosidase. By calculating the IC50, the flavonoids extracted from fermented sugarcane leaves exhibited stronger scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals than did those from the unfermented samples.

Key words: sugarcane leaves; solid-state fermentation; phenolics; cellulase; β-glucosidase; antioxidant activity

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