食品科学 ›› 2020, Vol. 41 ›› Issue (19): 132-139.doi: 10.7506/spkx1002-6630-20190905-074

• 食品工程 • 上一篇    下一篇

不同干燥方式对红花玉兰花蕾挥发油成分及抗氧化、抗菌活性的影响

程嘉莉,马江,肖爱华,朱仲龙,桑子阳,马履一   

  1. (1.北京林业大学林学院,省部共建森林培育与保护教育部重点实验室,北京 100083;2.湖北五峰土家族自治县林业科学研究所,湖北?宜昌 443413)
  • 出版日期:2020-10-15 发布日期:2020-10-23
  • 基金资助:
    林业公益性行业科研专项(201504704)

Effect of Different Drying Methods on Chemical Composition, Antioxidant and Antibacterial Activities of Essential Oils from Buds of Magnolia wufengensis

CHENG Jiali, MA Jiang, XIAO Aihua, ZHU Zhonglong, SANG Ziyang, MA Lüyi   

  1. (1. Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China; 2. Forestry Research Institute of Wufeng County, Yichang 443413, China)
  • Online:2020-10-15 Published:2020-10-23

摘要: 为探究干燥方式对红花玉兰花蕾挥发油萃取及抗氧化、抗菌活性的影响,采用超临界CO2萃取法分别萃取经冷冻干燥、阴干、晒干和55 ℃烘干处理的红花玉兰‘娇红1号’花蕾的挥发油,并通过气相色谱-质谱联用结合保留指数对挥发油进行定性和定量分析,同时通过测定1,1-二苯基-2-三硝基苯肼自由基清除率、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸阳离子自由基清除率和还原能力评价挥发油的抗氧化活性,采用滤纸片扩散法评价挥发油的抗菌活性。结果表明:冷冻干燥花蕾的挥发油萃取率最高,达(2.736±0.138)%;4 种干燥处理后的花蕾的挥发油共鉴定出49 种化合物,其中冷冻干燥鉴定出26 种,γ-谷甾醇和4H-xanthalongia的相对含量最高,晒干、55 ℃烘干、阴干分别鉴定出20、20 种和30 种,γ-谷甾醇和烃类的相对含量最高;挥发油的抗氧化活性由高到低依次为:冷冻干燥>阴干>晒干>55 ℃烘干,均具有广谱抑菌性。综合而言,红花玉兰花蕾经冷冻干燥处理后其挥发油萃取效果最佳。

关键词: 红花玉兰花蕾挥发油;超临界CO2萃取;气相色谱-质谱联用;抗氧化活性;抗菌活性

Abstract: In order to investigate the effect of drying methods for flower buds on the extraction, antioxidant and antibacterial activities of essential oil from Magnolia wufengensis, essential oils from the flower buds of Magnolia wufengensis ‘Jiaohong 1’ dried by freeze drying, shade drying, sun drying or oven drying at 55 ℃ were obtained by supercritical CO2 fluid extraction and analyzed for chemical composition by using gas chromatography-mass spectrometry (GC-MS) combined with retention index (RI). Furthermore, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging and reducing power assays were used to evaluate the antioxidant activity of the essential oils. Disk diffusion method was used to measure the antibacterial activity. The results indicated that the highest essential oil yield was obtained from the freeze-dried sample, up to (2.736 ± 0.138)%, and in total 49 compounds were identified in the essential oils from the four dried samples, among which, 26 compounds were found to be present in the essential oil from the freeze-dried sample, the most abundant being γ-sitosterol and 4H-xanthalongia; and 20, 20 and 30 compounds in the essential oils from the sun-dried, oven-dried and shade-dried samples, respectively, the most abundant being γ-sitosterol and hydrocarbon. The antioxidant activity of the four essential oils was in the decreasing order of freeze drying > shade drying > sun drying > oven drying, all of which had broad-spectrum antibacterial activity. In general, freeze drying is the best pretreatment method for extracting essential oils from the buds of M. wufengensis.

Key words: essential oils from buds of Magnolia wufengensis; supercritical CO2 fluid extraction; gas chromatography-mass spectrometry; antioxidant activity; antibacterial activity

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