纳米二氧化钛对小鼠亚慢性暴露的肠道微生物组和代谢组关联分析

doi:10.7506/spkx1002-6630-20220211-061

食品科学 ›› 2023, Vol. 44 ›› Issue (3): 162-169.doi: 10.7506/spkx1002-6630-20220211-061

纳米二氧化钛对小鼠亚慢性暴露的肠道微生物组和代谢组关联分析

赵化冰，杨晨，常慧敏，史婷婷，张冰洁，谭有兰，李凤珠，王洪彬，路福平

（1.天津科技大学?工业发酵微生物教育部重点实验室，天津 300457；2.天津科技大学?天津市工业微生物重点实验室，天津 300457；3.天津科技大学生物工程学院，工业酶国家工程实验室，天津 300457）

出版日期:2023-02-15 发布日期:2023-02-28
基金资助:
广东省重点领域研发计划项目（2019B020210001）

Correlation Analysis between Intestinal Microbiome and Metabolome of Mice Subchronically Exposed to Titanium Dioxide Nanoparticles

ZHAO Huabing, YANG Chen, CHANG Huimin, SHI Tingting, ZHANG Bingjie, TAN Youlan, LI Fengzhu, WANG Hongbin, LU Fuping

(1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China; 2. Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science & Technology, Tianjin 300457, China; 3. National Engineering Laboratory for Industrial Enzymes, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China)

Online:2023-02-15 Published:2023-02-28

摘要/Abstract

摘要： 基于微生物组学和代谢组学技术探讨纳米二氧化钛（TiO2）颗粒对C57BL/6小鼠肠道微生物和粪便代谢物的影响及组学间的关联性。设置对照组和实验组，对照组灌胃磷酸盐缓冲液，实验组以100 mg/（kg mb·d）纳米TiO2灌胃，连续暴露13 周。测定血糖、血脂等指标，并利用16S rDNA测序和气相色谱-质谱技术对肠道内容物进行分析，筛选出显著变化的微生物和差异代谢物。结果表明，与对照组相比，实验组小鼠空腹血糖水平显著上升（P＝0.032）。厚壁菌门（Firmicutes）、样棒菌属（Allobaculum）、乳杆菌属（Lactobacillus）、颤螺旋菌属（Oscillospira）相对丰度增加。差异代谢物与甘油酯类代谢、半乳糖代谢、色氨酸代谢通路有关。厚壁菌门细菌相对丰度与差异代谢物水平呈负相关，其他门类的细菌相对丰度与差异代谢物水平呈正相关。推断小鼠在纳米TiO2亚慢性暴露后，所产生的毒性作用可能与粪便中糖脂代谢异常相关。

关键词: 纳米二氧化钛；肠道；代谢组；微生物组

Abstract: The effects of titanium dioxide (TiO2) nanoparticles on the intestinal microbiome and fecal metabolome in C57BL/6 mice were investigated and the correlation between the intestinal microbiome and fecal metabolome was analyzed. A control group and an experimental group were set up, which were orally administered with phosphate buffered saline (PBS) and 100 (mg/kg mb·d) of TiO2 nanoparticles for 13 continuous weeks, respectively. Blood glucose and lipids were measured. Furthermore, 16S rDNA gene sequencing and gas chromatography-mass chromatography (GC-MS) were used to identified the significantly differential microorganisms and metabolites between the two groups. Our results showed that compared with the control group, fasting blood glucose was significantly increased in the experimental group (P = 0.032). The relative abundance of Firmicutes, Allobaculum, Lactobacillus, and Oscillospira was increased. The differential metabolites were associated with the glycerolipid, galactose and tryptophan metabolic pathways. Firmicutes bacteria were negatively correlated with the differential metabolites, while bacteria from other phyla were positively correlated with the differential metabolites. Therefore, we infer that the subchronic toxicity of TiO2 nanoparticles to mice may be associated with abnormal glycolipid metabolism in feces.

Key words: titanium dioxide nanoparticles; gut; metabolome; microbiome

中图分类号:

R994.4

赵化冰，杨晨，常慧敏，史婷婷，张冰洁，谭有兰，李凤珠，王洪彬，路福平. 纳米二氧化钛对小鼠亚慢性暴露的肠道微生物组和代谢组关联分析[J]. 食品科学, 2023, 44(3): 162-169.

ZHAO Huabing, YANG Chen, CHANG Huimin, SHI Tingting, ZHANG Bingjie, TAN Youlan, LI Fengzhu, WANG Hongbin, LU Fuping. Correlation Analysis between Intestinal Microbiome and Metabolome of Mice Subchronically Exposed to Titanium Dioxide Nanoparticles[J]. FOOD SCIENCE, 2023, 44(3): 162-169.

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纳米二氧化钛对小鼠亚慢性暴露的肠道微生物组和代谢组关联分析

Correlation Analysis between Intestinal Microbiome and Metabolome of Mice Subchronically Exposed to Titanium Dioxide Nanoparticles

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