落叶松阿拉伯半乳聚糖的Smith降解过程及其对食品相关免疫活性的影响

doi:10.7506/spkx1002-6630-20241022-140

食品科学 ›› 2025, Vol. 46 ›› Issue (13): 147-158.doi: 10.7506/spkx1002-6630-20241022-140

落叶松阿拉伯半乳聚糖的Smith降解过程及其对食品相关免疫活性的影响

刁心彦，戚慧敏，唐硕，陈亚楠，赖晨欢，黄曹兴，勇强

（1.南京林业大学化学工程学院，江苏省林业资源高效加工利用协同创新中心，江苏南京 210037；2.中华全国供销合作总社南京野生植物综合利用研究院，江苏南京 211111）

出版日期:2025-07-15 发布日期:2025-06-13
基金资助:
国家自然科学基金青年科学基金项目（32201514）

Smith Degradation of Arabinogalactan Derived from Larch and Its Potential as a Food Ingredient with Immunoenhancing Activity

DIAO Xinyan, QI Huimin, TANG Shuo, CHEN Yanan, LAI Chenhuan, HUANG Caoxing, YONG Qiang

(1. Jiangsu Collaborative Innovation Center for Efficient Processing and Utilization of Forestry Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Institute of Integrative Utilization of Wild Plants, All China Federation of Supply and Marketing Cooperatives, Nanjing 211111, China)

Online:2025-07-15 Published:2025-06-13

摘要/Abstract

摘要： 本实验以落叶松（Larix spp.）为对象，优化水提工艺（60 ℃、2 h），并利用XAD-16N树脂进行纯化处理，高效提取高纯度（93.79%）水溶性阿拉伯半乳聚糖（AGR），并解析其结构特性。通过Smith降解法，制得5 种降解产物，发现降解时间与产物分子质量呈负相关。体外免疫活性评估显示，特定降解产物（DAG1-10，阿拉伯糖、半乳糖质量比1∶11.15）能显著促进巨噬细胞分泌免疫介质（肿瘤坏死因子-α和白细胞介素-6），从而证实了其作为食品免疫增强剂的潜在价值。本研究为落叶松多糖在食品科学领域的高值化应用提供了理论支撑。

关键词: 落叶松, 阿拉伯半乳聚糖, Smith降解, 免疫活性, 高值化利用

Abstract: This study optimized the hot water extraction (at 60 ℃ for 2 h) of arabinogalactan from larch (Larix spp.) and purified it using XAD-16N resin. High-purity (93.79%) water-soluble arabinogalactan (AGR) was obtained and its structural characteristics were evaluated. After its Smith degradation, five products including DAG1-10, composed of arabinose and galactose at a mass ratio of 1:11.15, were obtained. A negative correlation existed between the degradation time and the molecular masses of the products. The in vitro immunoregulatory evaluation indicated that DAG1-10 significantly promoted the secretion of immune mediators such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by macrophages, thereby confirming its potential as an immunoenhancer. This research provides theoretical support for the high-value application of Larix spp. polysaccharides in the field of food science.

Key words: larch, arabinogalactan, Smith degradation, immunoenhancing activity, high-value utilization

中图分类号:

TS201.2

刁心彦, 戚慧敏, 唐硕, 陈亚楠, 赖晨欢, 黄曹兴, 勇强. 落叶松阿拉伯半乳聚糖的Smith降解过程及其对食品相关免疫活性的影响[J]. 食品科学, 2025, 46(13): 147-158.

DIAO Xinyan, QI Huimin, TANG Shuo, CHEN Yanan, LAI Chenhuan, HUANG Caoxing, YONG Qiang. Smith Degradation of Arabinogalactan Derived from Larch and Its Potential as a Food Ingredient with Immunoenhancing Activity[J]. FOOD SCIENCE, 2025, 46(13): 147-158.

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落叶松阿拉伯半乳聚糖的Smith降解过程及其对食品相关免疫活性的影响

Smith Degradation of Arabinogalactan Derived from Larch and Its Potential as a Food Ingredient with Immunoenhancing Activity

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