基于MOFs固定化漆酶对植物油中黄曲霉毒素B1和玉米赤霉烯酮的降解

摘要/Abstract

摘要： 黄曲霉毒素（AFB1）和玉米赤霉烯酮（ZEN）是植物油中主要的污染物之一，传统的物理与化学去除效果有限，仍需开发绿色、高效、安全的霉菌毒素脱除技术。本研究以Fe-MOFs材料为载体，以漆酶（Lac）为目标物，通过原位封装法合成Lac@Fe-BTC生物复合材料，并探究其对食用油中AFB1和ZEN的去除作用及机制。结果表明， MOFs固定化后的漆酶热稳定性提高，其对花生油中AFB1和ZEN的去除率分别为71.64%和60.98%，显著高于游离漆酶。此外，对真菌毒素及其降解产物进行结构鉴定和斑马鱼毒理实验，证实了降解产物毒性显著降低。与游离漆酶相比，该方法展现出更高的真菌毒素去除速率、稳定性和可重复使用性，因此在真菌毒素脱除领域具有良好的应用前景。

关键词: 关键词：固定化漆酶, 金属有机骨架材料, 花生油, 真菌毒素降解, 安全性

Abstract: Aflatoxin B1 (AFB1) and zearalenone (ZEN) are major contaminants in vegetable oils. Conventional physical and chemical detoxification methods demonstrate limited efficacy. Consequently, developing green, efficient, and safe mycotoxin removal technologies is critically needed. This study employed Fe-MOFs as carriers to immobilize laccase (Lac) via in-situ encapsulation, synthesizing a Lac@Fe-BTC biocomposite. Then the removal efficacy and underlying mechanisms of this composite for AFB1 and ZEN in edible oils were then investigated.Results indicated that Laccase immobilization on MOFs enhanced thermal stability and achieved AFB1 and ZEN removal rates of 71.64% and 60.98% in peanut oil, respectively, significantly exceeding those of free laccase.Furthermore, structural characterization of the mycotoxins and their degradation products, alongside zebrafish toxicity evaluation, demonstrated a significant reduction in their toxicity.Compared to free laccase, the immobilized enzyme demonstrated superior efficiency, stability, and reusability for mycotoxin removal. Therefore, this approach exhibits promising potential for mycotoxin decontamination applications.

Key words: Keywords: Immobilized laccase, Metal-organic frameworks (MOFs), Peanut oil, Mycotoxins degradation, Safety

中图分类号:

TS201. 2

王梦瑶孙淑敏. 基于MOFs固定化漆酶对植物油中黄曲霉毒素B1和玉米赤霉烯酮的降解[J]. 食品科学.

Meng-Yao WANG. Degradation of Aflatoxin B1 and Zearalenone in Vegetable Oils Using MOF-Immobilized Laccase: A Comparative Study[J]. FOOD SCIENCE.

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