食品科学 ›› 2010, Vol. 31 ›› Issue (21): 137-141.doi: 10.7506/spkx1002-6630-201021031

• 基础研究 • 上一篇    下一篇

酶改性对高温变性豆粕溶解性的影响

任为聪1,程建军1 ,* ,张智宇1,赵伟华1,江连洲1 , 2   

  1. 1.东北农业大学食品学院
    2.国家大豆工程技术研究中心
  • 收稿日期:2010-08-10 修回日期:2010-10-23 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 程建军 E-mail:cheng577@163.com
  • 基金资助:

    黑龙江省科技计划项目(GB08B401-02)

Effect of Enzymatic Modification on Solubility of High-temperature Denatured Soybean Meal

REN Wei-cong1,CHENG Jian-jun1,*,ZHANG Zhi-yu1,ZHAO Wei-hua1,JIANG Lian-zhou1,2   

  1. 1. College of Food Science, Northeast Agricultural University, Harbin 150030, China;
    2. National Soybean Engineering and Technique Research Center, Harbin 150030, China
  • Received:2010-08-10 Revised:2010-10-23 Online:2010-11-15 Published:2010-12-29
  • Contact: CHENG Jian-jun E-mail:cheng577@163.com

摘要:

采用碱性蛋白酶改性提高高温变性豆粕的溶解性。以氮溶解指数(NSI)为指标,考察pH 值、底物质量浓度、加酶量、温度、时间对高温变性豆粕NSI 的影响。通过单因素和响应面试验确定优化酶解高温变性豆粕的工艺条件,在pH9.0、底物质量浓度8.56g/100mL、加酶量13004.69U/g pro、温度59.10℃、时间20.47min 时,水解度(DH)虽然为15.86%,但NSI 值从21.24% 达到了92.58%。

关键词: 酶法改性, 高温变性豆粕, 响应面, NSI

Abstract:

The effect of alkaline portease modification on the nitrogen solubility index (NSI) of high-temperature denatured soybean meal was investigated. The optimal conditions for the enzymatic hydrolysis of high-temperature denatured soybean meal were explored by single factor method and response surface analysis. The optimal enzymatic hydrolysis conditions were found to be: substrate concentration, 8.56 g/100mL; enzyme loading, 13004.69 U/g pro; hydrolysis pH 9.0, hydrolysis temperature, 59.10 ℃; and hydrolysis time, 20.47 min. The degree of hydrolysis (DH) was 15.86% under the optimal hydrolysis conditions, and the NSI was 92.58%, much higher than that before the hydrolysis of 21.24%.

Key words: enzymatic modification, high-temperature denatured soybean meal, response surface analysis, NSI

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