Abstract:

The volatile compounds of Thlaspi arvense L. were isolated with steam distillation, and identified by capillary GCMS method and quantified by area normalization method. The binding forms of selenium in Thlaspi arvense L. was analyzed using microwave digestion and atomic fluorescence spectrometry (AFS). A total of 18 volatile components were identified and determined, accounting for 92.47% of the total peak area, of which, the main volatile compound was delphinic acid, followed in turn by 3-methylvaleric acid, 5-bromothiophene-2-carboxamide, 3,5-diamino-1,2,4-triazole, methyl formate, and formic hydrazide. In addition, an amount of several elements such as nitrogen, phosphorus, sulphur and halogens was also found in Thlaspi arvense L. Organic selenium was found to be the main form of selenium in Thlaspi arvense L.. Among the forms of organic selenium, protein-bound selenium constituted a major fraction, and selenium was also found to be bound to other substances such as nonprotein substances, polysaccharides, volatile compounds and nucleic acids.

Key words: Thlaspi arvense L., volatile constituent, GC-MS, selenium, binding form, atomic fluorescence spectrometry (AFS)