Abstract:

Characteristics of liquid cultivation of four strains of Ganoderma lucidum and one strain of Hypsizygus marmorens were investigated by using oat emulsion as the medium. During the cultivation, pH, intracellular polysaccharide (IPS), extracellular polysaccharide (EPS), cell dry weight, and β-glucan content were measured every day. Characteristics of the fermentation process were analyzed according to the change of these parameters. A beverage was developed on the basis of cell-free liquid culture of the Ganoderma lucidum strain, LINGZHI3. All of four Ganoderma lucidum strains grew well in oat emulsion and produced a large amount of IPS and EPS. The profiles of IPS exhibited a good match with dried cell weight (DCW) and IPS reached a maximum level on the 6th day, while EPS on the 5th, 6th and 7th day for four strains of Ganoderma lucidum. EPS production of Hypsizygus marmorens exhibited a decreasing trend during the whole cultivation. The highest IPS did not match with the highest DCW and exhibited a negative relationship. These results indicate that oat is a good medium for the production of Ganoderma lucidum polysaccharides by submerged liquid cultivation, but not good for Hypsizygus marmorens polysaccharides. A high quality beverage was developed through adding 1.5 g/L CMC-Na to clarify fermented broth of Ganoderma lucidum. This beverage exhibited a good balance between sweet and sour taste, stable texture, clear appearance and plentiful nutrients, and also contained 103 g/L polysaccharides, 1 g/L total acid, 11.6 g/L amino acids and 40 g/L soluble solids.

Key words: oat, Ganoderma lucidum, Hypsizygus marmorens, liquid cultivation, polysaccharide